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Luciferase Reporter Cell Lines for Monitoring
TF Binding and Activation
Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based reporter assays. At Signosis, we help researchers save time and labor in generating and validating stable cell lines so scientists can spend more effort on solving the big questions.
Our Luciferase Reporter Stable cell lines are genetically engineered to express luciferase during the activation of specific TFs Binding and Activation to provide an efficient approach to study and monitor various cellular processes, including gene regulation and signaling pathways.
We offer a wide selection of validated Firefly luciferase reporter cell lines that measure transcription factor activity as a read-out for various signaling pathways.
We also offer Gaussia luciferase reporter cell lines, a special type of luciferase that is naturally secreted from cells, which allows for the easy measurement of reporter activity directly in the media without any need to harvest the cells.
Principle
TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation
Benefits
High Sensitivity and Responsiveness
Each cell line is validated to induce a strong reporter signal in response to stimuli, and is highly responsive to our Firefly Luciferase Substrate.
Mycoplasma Free
All cell lines have been tested negative for mycoplasma.
Consistent
The TF reporter construct is stably integrated into the genome to avoid cell-to-cell variations in experiments.
We offer a highly sensitive Firefly Luciferase Substrate, which can accurately measure firefly luciferase activity in cells.
Efficient
Cell lines can be used right away for experiments to study different signaling pathways.
Products
Signaling Pathways
Cell Lines
