Associated Products

Research Solution Availability

Luciferase Substrate

Benefits

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High signal and responsive

Produces strong and stable light signal.

Simple, easy to use

One-step solutions

Ready to use without any prior preparation or mixing needed

Compatible

Works with all Signosis luciferase products and third-party firefly luciferase systems

Cost Effective

Less $ spent per reaction compared to our competitors

EGFR Vectors

Principle

Mutations in the epidermal growth factor receptor (EGFR) gene are commonly observed in many type of cancers including lung, colorectal, head and neck and breast cancer. These mutations play an important role in particular in non-small-cell lung cancer as they can be not only therapeutic target but also a predictive tool for response to EGFR tyrosine kinase inhibitors such as gefitinib and erlotinib. Cell lines have played crucial roles in the identification and characterization of these driver mutations.

Signosis has developed expression vectors for the most common EGFR mutations harboring FLAG marker with high expression suitable for your cancer research. We also accept any other customized EGFR mutation for your research need, contact us for more information.

pCMV-EGFR

Benefits

A panel of EGFR Mutant Vectors

The panel includes more than 12 ready-to-use EGFR mutants.

DYKDDDDK-Tag for Easy Detection

This tag is engineered to downstream of EGFR gene to facilitate Western blot

Perfect for Stable Cell Line Establishment

The hygromycin-resistant gene is embedded in the vector for stable cell line establishment.

Custom Service Available

We also offer the custom service with the same list price. If you have any mutants in your mind, please let us know.

Cell Vialbility Assay (CVC)

Principle

Cell Viability/Cytotoxicity (CVC) Reagent is a colorimetric WST-8 based assay that provides a simple and accurate method to measure cell proliferation. This is a tetrazolium reduction assays based on the cleavage of the tetrazolium salt WST-8 to water-soluble, orange formazan dye by cellular mitochondrial dehydrogenases (Fig.1). Intensity of dye is a direct proportion of increased activity of the mitochondrial dehydrogenases and therefore number of viable cells which can be quantified by measuring the absorbance at 450 nm.

Ready to use CVC Reagent allow for rapid, sensitive and reliable method for measuring cell viability/cytotoxicity directly from a 96-well plate without any additional processing.