TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression.  The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites.  There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways.  Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive Gaussia Luciferase gene expression.  

The Gaussia Luciferase is a gene from the copepod Gaussia princeps, which is a small, naturally secreted luciferase of approximately 20kDa. This luciferase is naturally secreted from the cells, which allows for the measurement of reporter activity in the media without harming the cells. 

Gaussia luciferase requires a coelenterazine-based luciferase substrate for reading and should not be used with firefly luciferase substrate.