Nuclear receptors (NRs) are a superfamily of transcription factors that regulate key aspects of development, metabolism and disease. In general, they activate the transcription of sets of genes in response to the binding of cognate ligands. The classic modular domain organization of NRs includes a variable N-terminal domain (NTD), a centrally located zinc-finger DNA-binding domain (DBD) and a C-terminal ligand-binding domain (LBD).
The GAL4-UAS system is a powerful technique for studying gene expression and function. It has also been adapted to study nuclear receptor chemical-binding functions. Signosis NR LBD-driven GAL4 reporter stable cell lines is a reliable, rapid, and sensitive method for analyzing the intracellular status of NRs upon exposure to drug candidates or other stimuli. These functional assays allow for measurement of receptor activation or inhibition by compounds and can be used to determine compound potency and selectivity.
- Highly specific–the responsiveness of this stable cell line is driven by the specific ligand binding domain with low/no cross reactivity with other members of nuclear receptor.
- Less toxic – The chimeric receptors display less toxic effects on the cells.
- Highly sensitive – More than 50 fold induction in response to the corresponding stimuli.
- Routine mycoplasma testing - All cell lines tested negative for mycoplasma.
|Product Name||Catalog #||Price (FP)*||Catalog #||Price (NP)**||Qty|
|GR LBD-driven GAL4 reporter HEK 293 stable cell line||SL-3001-FP||$6,000||SL-3001-NP||$3,000|
- * For Profit (FP) price is for non-academic, private companies and institutes
- ** Non Profit (NP) price is for academic, non profit organizations and institutes