Xenobiotic Stress

Xenobiotic response element (XRE) is a DNA binding sequence at the upstream of inducible target genes for the transcription factor aryl hydrocarbon receptor (AhR) that is responsible for regulation of stress pathway in eukaryotic cells. The AhR regulates response to xenobiotic toxicity from variety of chemicals like 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The AhR functions as a ligand-activated transcription factor that binds to a canonical xenobiotic response element (XRE) in association with the heterodimerization partner, the AhR nuclear translocator (Arnt) protein.

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Signosis has established XRE luciferase reporter HEK293 stable cell line that has been stably transfected with XRE-luciferase reporter vector and can be used for studying TCDD toxicity and most cellular stress-related responses. The cell line was established by transfection using a XRE-luciferase reporter vector, which contains 4 repeats of Xenobiotic response element (XRE), a minimal promoter upstream of the firefly luciferase coding region, along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for TCDD-induced luciferase activity.

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