top of page

TF Luciferase Reporter Vectors

Measure transcription factor activation in cells using our extensive collection of over 100 firefly luciferase-based reporter vectors. Each pTF-Luc vector is designed to provide a quantitative readout of TF activity, and we offer custom services to create vectors for any unlisted transcription factors or selection markers.

Principle 

Each vector is designed to monitor the activity of a single transcription factor (TF), with at least four copies of a cis-acting enhancer element placed upstream of a minimal TATA promoter. Upon transfection and stimulation, activated TFs bind to the enhancer, triggering luciferase gene expression. The resulting chemiluminescent signal directly reflects TF activity.

To support stable cell line generation, we offer over 50 HygroSelect and 50 NeoSelect vectors, each with hygromycin or neomycin resistance and GFP for visual selection. For dual-pathway monitoring in one cell line, use one vector with hygromycin and one with neomycin selection.

Signosis also provides custom vector services for unlisted TFs or selection markers. For more details or recommendations, contact us at 408-747-0771 or info@signosisinc.com.
TF Luciferase Vector.png
Screenshot 2025-04-10 142114.png

Basic TF  Luciferase Reporter Vectors

Cost Effective solutions TF Reporter Vectors without any selection marker, suitable for transient transfection.

Can be used for the creation of stable cell lines but will require co-transfection with a resistance vector of your choice.

Screenshot 2025-04-10 142449.png

HygroSelect TF Luciferase Reporter Vectors with Optional GFP as a Selection Marker

TF Reporter Vectors with the hygromycin resistance gene included with optional GFP as a selection marker. 

Screenshot 2025-04-10 142647.png

NeoSelect TF Luciferase Reporter Vectors with GFP

TF Reporter Vectors that include neomycin resistance gene along with a GFP expression gene. The expression of GFP suitable for both selection purposes and for confirming transfection efficiency.

Product Categories

Benefits

Simplified Stable Cell Line Generation 

Integrated selection markers (hygromycin or G418 resistance) for easy antibiotic selection.

Flexible Experimental Design

Two sets of vectors enable stable expression of two different TFs in the same cell line.

Dual Selection

Hygromycin and Neomycin resistant gene dual selection.

Highly Sensitive Substrate for Detection

Our proprietary Firefly Luciferase Substrate (SKU# LUC100) is designed for optimal detection of firefly luciferase expression, seamlessly compatible with our TF Luciferase Reporter Vectors

Visualization

Some vectors include GFP, allowing fluorescence-based tracking of transfected cells.

Customizable Solutions

Custom vector modifications and stable cell line services are available upon request.

bottom of page