The Luciferase Reporter Vectors have been specially constructed to report the binding activity of a single TF. Multiple copies (at least four) of the cis-acting enhancer element have been inserted into each vector upstream of a minimal TA promoter. Upon vector transfection and cell stimulation, the activated TF is bound to the enhancer element and initiate the transcription of downstream luciferase reporter gene. The chemiluminescent measurement of luciferase activity is proportional to the amount of expressed enzyme and thus the binding activity of the targeted TF. 

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Transcription factor (TF) luciferase reporter vectors with hygromycin and/or GFP selection have been recently launched, containing a luciferase gene under the control of multiple repeats of TF consensus binding sequence and TATA minimal promoter, allowing researchers to monitor the activity of that promoter in different experimental conditions. In order to facilitate stable cell line generation, the vectors have been added selection markers, hygromycin resistance or GFP (Green Fluorescent Protein).  Hygromycin resistance allows for selection using the antibiotic hygromycin, while GFP provides a visible marker for identifying transfected cells.