Description(s):
Epidermal growth factor receptor (EGFR) is a cell-surface receptor with intrinsic intracellular protein-tyrosine kinase (TK) activity. Ligand binding induces EGFR dimerization and phosphorylation, leading to the activation of the EGFR signaling pathway. In several malignancies such as non-small cell lung cancer (NSCLC), EGFR signaling is deregulated due to mutations in EGFR, which results in uncontrolled proliferation and migration of tumor cells. EGFR mutations can lead to oncogene-addicted cancers, where the tumor cells depend on the mutated EGFR for cell survival and the malignant phenotype.
 
EL-001 is a stable cell line established with an empty vector as a control for three stably expressing HA-tagged EGFR Ba/F3 cell lines; WT EGFR, L858R EGFR mutant, L858R/T790M EGFR double mutant. These cell lines can be used to study the molecular mechanism underlying the susceptibility of tumors to the drugs (i.e. gefitinib and erlotinib) as well as screening and validating new TKIs.