Principle 

Signosis' Magnetic Beads ChIP Assay improves traditional ChIP by enabling more efficient and sensitive detection of transcription factor complexes. The assay involves four steps: (1) cross-linking proteins to DNA, (2) chromatin fragmentation, (3) immunoprecipitation, and (4) target identification.

Key improvements include:

• Dual cross-linking: Combines formaldehyde (short spacer arm) with a second reagent (long spacer arm) to capture both DNA-bound TFs and associated coactivators/corepressors.

• MNase digestion: Efficiently fragments chromatin into mononucleosomes (~500 bp), enhancing sensitivity and reproducibility.

• Streamlined DNA recovery: Uses a one-step Chelex-100 method to release DNA, eliminating proteinase K digestion and extra purification steps.

These enhancements make the assay faster, more reliable, and ideal for studying protein-DNA interactions in detail.