The Nuclear Extraction Kit is used for preparation of nuclear extracts, which often need for studying transcription factors. The Nuclear Extraction Kit contains reagents for the preparation of 200 nuclear extracts from culture cells in 6-well plate and 50 nuclear extracts from cultured cells in 100-mm culture dishes. The kit can also be used for nuclear extraction of soft tissue samples (brain, kidney, liver, etc), however there may be additional steps required to release cells from tissues.