USF-1 EMSA (Electrophoretic-Mobility Shift Assay) kit is used for monitoring USF-1(Upstream transcription factor 1 ) DNA binding activities and activation. Upon activation, USF-1 can recognize and interact with the unique DNA binding sequence and form protein/DNA complex, which can be separated from free DNA due to the differences in their electrophoretic mobility in native (non-denaturing) polyacrylamide gels.