The Triglyceride Assay Kit utilizes a series of enzyme reactions to measure triglyceride levels in samples. First, the triglyceride is hydrolyzed by lipoprotein lipase into glycerol. Then, the glycerol is converted to glycerol 3 phosphate by glycerol kinase. Lastly, the glycerol 3 phosphate is oxidized by glycerol 3 phosphate oxidase, which produces hydrogen peroxide. The triglyceride level in the sample is determined by quantifying the hydrogen peroxide generated by the enzyme reaction with a fluorogenic probe that can be measured with a spectrophotometer.