Stem cells are important cells characterized by their ability to self-renew or differentiate into many cell types, which is governed by intracellular signaling pathway and transcription regulation. Hence, transcription regulation plays a determinant role in conferring cellular identity and function. The step-wise maturation of stem cells into terminally differentiated cell types requires the timely activation of a cascade of transcription programs governed by lineage-specifying transcription factors. Multiple transcription factors and target genes have been widely reported to associate with stem cell self-renewal and pluripotency, including EGR1, OCT4, FOXD3, FOXO, Nanog, SOX2, SOX18, ETS, GLI, KLF4, MEF2, Myc, RUNX1, Pax6, TCF/LEF and GATA. Analyzing the activities of these TFs can provide valuable clues in the study and manipulation of stem cells. Signosis has developed Stem Cell Transcription Factor Activation Plate Array to analyze the activities of 16 stem cell-specific TFs simultaneously in mammalian samples. The assay can be used with the whole cell lysis from 1000-10000 cells.
 
The data for the initial image of FA-1003 was derived from our customer's publication, NIH. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113365/).