Forkhead box A1 (FOXA1) is a transcription factor that plays a critical role in the regulation of gene expression related to development, differentiation, and cancer biology. The FOXA1 luciferase reporter vector is designed to study the transcriptional activity of FOXA1. The vector contains a FOXA1 DNA consensus binding site and TA minimal promoter upstream of a firefly luciferase reporter gene, which can specifically report FOXA1 activity while maintaining a low basal level of transcription.