Oct-1 EMSA (Electrophoretic-Mobility Shift Assay) kit is used for monitoring Oct-1 ( POU domain, class 2, transcription factor ) DNA binding activities and activation. Upon activation, Oct-1 can recognize and interact with the unique DNA binding sequence and form protein/DNA complex, which can be separated from free DNA due to the differences in their electrophoretic mobility in native (non-denaturing) polyacrylamide gels.