The Lipid Peroxidation MDA Assay utilizes thiobarbituric acid (TBA) to detect malondialdehyde (MDA) in biological samples. MDA is a marker for oxidative stress and forms from the lipid peroxidation of polyunsaturated fatty acids. When TBA reacts with MDA, a fluorescent red product is formed that can be measured spectrophotometrically at an absorbance of 532 nM.