Angiogenesis provides the crucial link between the avascular and vascular states and is a key event for sustained tumor growth and cancer progression. Angiogenesis as a biological switch process is governed by numerous pro- and anti-angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGFb), epidermal growth factor (EGF), and transforming growth factor-beta (TGFβ). The mechanism of action of each of these factors is different, as are their origin and the stimuli for their production. The angiogenic switch refers to the balance between pro- and anti- angiogenic factors. Therefore, profiling of these factors is critical to understanding angiogenesis. Signosis' Angiogenesis ELISA Strip I Profiling Assay allows simultaneously profiling 8 angiogenesis cytokines. Each well of the strip is coated with a primary antibody against a specific angiogenesis protein and total 8 wells of a strip target 8 different proteins. The difference of these proteins between two samples can be determined through data comparison.