HIF EMSA (Electrophoretic-Mobility Shift Assay) kit is used for monitoring HIF (Hypoxia inducible factor) DNA binding activities and activation. Upon activation, HIF can recognize and interact with the unique DNA binding sequence and form protein/DNA complex, which can be separated from free DNA due to the differences in their electrophoretic mobility in native (non-denaturing) polyacrylamide gels.