ER Responsive Luciferase Reporter MDA-MB-453 Stable Cell Line is derived from human breast cancer, and stably express firefly luciferase reporter gene under the control of the ER response element. This cell line is an ideal cellular model for monitoring the activation of Estrogen Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
 
Estrogen receptor (ER) belongs to nuclear receptor family and plays a widespread role in human physiology and in the development or progression of numerous diseases. In response to estrogen stimulation, estrogen bound receptor in the nucleus dimerizes and binds to specific response elements known as estrogen response elements (EREs) located in the promoters of target genes and regulates their gene expression.
 
Signosis has established MDA-MB-453 ER luciferase reporter stable cell line, in which the ERE and reporter luciferase gene are consistently expressed in the cell line to facilitate the screening and study. This stable cell line can provide a sensitive, responsive, and rapid in vitro system to detect and measure substances with potential (anti-)estrogenic activity.
 
Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation.