Bsu DNA Polymerase, Large Fragment is a DNA polymerase derived from Bacillus subtilis (Bsu) that is expressed through recombinant expression in Escherichia coli. This enzyme is a deletion mutant (1-296 aa deletion) of Bsu DNA Polymerase I, which exhibits 5'-3' DNA polymerase activity but lacks 3'-5' and 5'-3' exonuclease activities. The Bsu DNA Polymerase Large Fragment possesses strand displacement activity and is commonly used in Recombinase Polymerase Amplification (RPA), with the typical amplification temperature being 37°C.
**This product does not contain endonucleases, exonucleases, or ribonucleases.
Product Name: Bsu DNA Polymerase, Large Fragment Kit
Contents:
- Bsu DNA Polymerase, Large Fragment (BSUP-150, BSUP-750)
- 10× Bsu Reaction Buffer
Specifications:
- Bsu DNA Polymerase Size: 50µL with 150µL of 10x BSU Reaction Buffer (BSUP-150), 250µL with 750 µL of 10x BSU Reaction Buffer (BSUP-750)
- Bsu DNA Polymerase Units: 5 U/µL
- Bsu DNA Polymerase Reactions: 50, 250 reactions
Product Applications: Isothermal amplification methods like RPA; RPA strand displacement DNA synthesis; Random primer labeling; cDNA second strand synthesis; Single dA tailing.
Storage Conditions: Store at -20°C for two years; transport at ≤0°C.
Enzyme Storage Buffer
40 mM Tris-HCl (pH 7.5)
50 mM NaCl
0.1 mM EDTA
1 mM DTT
50% glycerol.
10×Bsu Buffer
100mM Tris-HCl（pH7.9） |
500mM NaCl |
100mM MgCl2 |
10mM DTT |
0.05% Proclin150 |
Definition of Activity: One unit (U) of activity is defined as the amount of enzyme required to incorporate 10 nmol of total nucleotides into an acid-insoluble form within 30 minutes at 37°C. Inactivation Incubate at 75°C for 20 minutes.
Quality Control Exonuclease Activity Assay: Incubate 5 U of the enzyme with 1 μg of Hind III-treated λDNA at 37°C for 10 hours. No changes should occur in the DNA electrophoresis pattern.
Endonuclease Activity Assay: Incubate 5 U of the enzyme with 1 μg of pUC19 plasmid DNA at 37°C for 1 hour. No changes should occur in the DNA electrophoresis pattern.
Ribonuclease Activity Assay: Incubate 5 U of the enzyme with 1 μg of 16S, 23S rRNA at 37°C for 1 hour. No changes should occur in the RNA electrophoresis pattern.
Please see manual for more information.
