Amino acid deprivation-AARE Luciferase Reporter MEF Stable Cell Line is derived from murine embryonic fibroblast, and stably express firefly luciferase reporter gene under the control of AARE (Amino acid response element). This cell line is an ideal cellular model for monitoring Amino acid response Signaling Pathway triggered by amino acid starvation or stimuli treatment.
 
Besides the central role of amino acids as building blocks of proteins they are also involved in other cellular functions like transcription, mRNA maturation, translation, mRNA turnover, autophagy and metabolism. Lack of amino acids (deprivation) will trigger the amino acid response (AAR) pathway largely through signal transduction and mediated through altered transcriptional activity of specific genes, including eIF2α, Asns, Atf4 and Chop.
 
Signosis has developed a luciferase reporter stable cell line under control of AARE (Amino acid response element) that can be easily used to monitor activation of Amino acid response Signaling Pathway.
 
Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation.