p53 Pathway

The p53 pathway plays a crucial role in effective tumor suppression because of its central function in cell cycle regulation, DNA repair, cellular senescence, and apoptosis, which can be used for potentially develop new drug therapies against cancer. Upon activation by DNA damage, oncogene activation, or hypoxia, p53 binds to its DNA recognition site on the promoter regions of the target genes and regulate the gene expression.  Signosis has established p53 luciferase reporter RKO stable cell line, in which luciferase activity is specifically associated with the activity of p53. Therefore, the cell line can be used as a reporter system for monitoring the activation of p53 triggered by stimuli treatment, enforced gene expression and gene knockdown.

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The cell line was established by transfection of p53 luciferase reporter vector along with G418 expression vector followed by G418 selection. The G418 resistant clones were subsequently screened for etoposide-induced luciferase activity. The clone with the highest fold induction (35 fold) was selected and expanded to produce this stable cell line.