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miRNA Northern Blot Assays
Detecting microRNAs (miRNAs) has never been more efficient or precise. Signosis’ miRNA High-Sensitivity Northern Blot Assay offers a non-radioactive, highly sensitive solution for analyzing miRNA expression. Using biotin-labeled detection probes plus signal amplification, this assay achieves sensitivity 100 times greater than conventional methods, making it ideal for detecting both high- and low-expressing miRNAs. With its ability to differentiate mature miRNAs from precursors and validate microarray or PCR results, this assay is an indispensable tool for advancing gene regulation and miRNA pathway research. Backed by excellent customer feedback and many citations, it’s a trusted choice for researchers worldwide.
Principle
RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe. This regular Northern blot assay is suitable for most moderate to high expressors of miRNA. The Highly Sensitive Northern Blot (NB-1001 and HP-XXXX) is needed to analyze low expressors of miRNA. The biotin-labeled probes contain two moieties: complementary sequence of the miRNA and a tag sequence. The tag sequence is then detected by an amplifier enriched with biotin molecules.
Assay | Product | SKU | Price (USD$) |
---|---|---|---|
Northern Blot | NB-0002 | 481 | |
Northern Blot | NB-1002 | 511 |