Metabolic Stress

Besides the central role of amino acids as building blocks of proteins they are also involved in other cellular functions like transcription, mRNA maturation, translation, mRNA turnover, autophagy and metabolism. Lack of amino acids (deprivation) will trigger the amino acid response (AAR) pathway largely through signal transduction and mediated through altered transcriptional activity of specific genes, including eIF2α, Asns, Atf4 and Chop.

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Signosis has developed a luciferase reporter stable cell line under control of AARE (Amino acid response element) that can be easily used to monitor activation of Amino acid response Signaling Pathway.