MAPK/JNK/AP-1

AP-1 plays a key role in the control of cell proliferation, differentiation, transformation, survival and apoptosis.  cJun and Fos dimerize to form AP-1 and this protein complex selectively binds to DNA sequences containing the TPA-responsive elements (TREs) to regulate gene expression.  Many stimuli, both physiological and pathological, can regulate AP-1 activity, including TPA, serum, growth factors, cytokines, stress signals, infections, and oncoproteins, TNF and IL-1.  AP-1 activity can be used as readout for JNK, ERK, or MAPK kinase signaling pathway. 

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Signosis has developed AP-1 luciferase reporter HeLa stable cell line by transducing cells with baculovirus containing both AP-1 luciferase reporter and hygromycin expression cassette.  The hygromycin resistant clones were subsequently screened for PMA-induced luciferase activity.  

The cell line can be used as a reporter system for monitoring the activity of AP-1 triggered by stimuli treatment, such as TNFa, IL-1, gene overexpression and gene knockdown.  The cells contain no viral particles and require handling at biosafety level 1 protocol.