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JAK2 Mutants Stable Cell Lines

Tyrosine Kinases

Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based assays.  Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis. 

At Signosis, we help researchers save time and labor in generating and validating stable cell lines so scientists can spend more effort on solving the big questions.  We offer a wide selection of validated luciferase reporter cell lines that measure transcription factor activity as a read-out for various signaling pathways.

Principle

The cell line was generated with expression vector in combined with hygromycin resistant and or puromycin resistant as well as GFP expression into BaF3 cells, and the single clones have been selected with hygromycin treatment, GFP expression. The positive clone has been confirmed with PCR-sequencing and Western blot with anti-JAK antibody.

Benefits

Virus Free

 

Our engineered expression vectors are delivered into cells through electroporation instead of viral gene delivery.

Double Selection

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Single clones are selected with GFP and hygromycin.

Comprehensive Validation

The selected clones have been validated with PCR-sequencing and Western blot analysis.

Functional Analysis

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​Proliferation tests have been performed on the cell lines with their corresponding inhibitors.

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