Glucocorticoid Receptor/GR

The Glucocorticoid receptor (GR) is a major player in development, metabolism and immune response.  When activated by stimuli, GR translocates from the cytoplasm into the nucleus, and then binds a DNA recognition site to regulate gene expression.  In addition, activated GR can transrepress other transcription factors that have been misregulated in cancer and other diseases.

The cell line was established by transfection of a GR firefly luciferase reporter vector along with G418 expression vector followed by G418 selection. The G418 resistant clones were subsequently screened for DEX-induced luciferase activity. The clone with the highest fold induction was selected and expanded to produce this stable cell line.

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