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EMSA

Signosis offers over 100 non-radioactive EMSA kits that simplify protein-DNA interaction studies. Each kit includes biotin-prelabeled probes and all necessary reagents, ensuring a safe, sensitive, and efficient workflow for analyzing transcription factor binding and gene regulation.

Principle 

When a transcription factor (TF) is activated, it binds to a biotin-labeled probe designed based on its consensus binding sequence. This TF/probe complex, when run through electrophoresis, migrates slower than the free probe, resulting in a distinguishable shifted band. By comparing the density of these shifted bands across different samples, variations in TF activation levels can be easily determined.
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Products

Target
Product
SKU
Price (USD$)
YY1

YY1 EMSA Kit

GS-0051
535
WT1

WT1 EMSA Kit

GS-0071
535
VDR

VDR EMSA Kit

GS-0053
535
USF-1

USF-1 EMSA Kit

GS-0052
535
TR

TR EMSA Kit

GS-0050
535
TFIID

TFIID EMSA Kit

GS-0049
535
TFE3

TFE3 EMSA Kit

GS-0087
535
TCF/LEF

TCF/LEF EMSA Kit

GS-0048
535
Stat6

Stat6 EMSA Kit

GS-0047
535
Stat5

Stat5 EMSA Kit

GS-0046
535
Stat4

Stat4 EMSA Kit

GS-0045
535
Stat3

Stat3 EMSA Kit

GS-0044
535
Stat1

Stat1 EMSA Kit

GS-0043
535
SRY

SRY EMSA Kit

GS-0074
535
SRF

SRF EMSA Kit

GS-0041
535
Sp1

Sp1 EMSA Kit

GS-0040
535
SOX9

SOX9 EMSA Kit

GS-0059
535
SOX5

SOX5 EMSA Kit

GS-0058
535
SOX2

SOX2 EMSA Kit

GS-0060
535
SOX18

SOX18 EMSA Kit

GS-0057
535
Snail

Snail EMSA Kit

GS-0089
535
SMUC

SMUC EMSA Kit

GS-0078
535
SMAD (MADH)

SMAD (MADH) EMSA Kit

GS-0039
535
SATB1

SATB1 EMSA Kit

GS-0042
535
RXR

RXR EMSA Kit

GS-0081
535
RUNX

RUNX EMSA Kit

GS-0080
535
ROR(RZR)

ROR(RZR) EMSA Kit

GS-0092
535
RB site

RB site EMSA Kit

GS-0073
535
PXR

PXR EMSA Kit

GS-0038
535
Prox1

Prox1 EMSA Kit

GS-0056
535
PR

PR EMSA Kit

GS-0020
535
PPAR

PPAR EMSA Kit

GS-0037
535
PLAG1

PLAG1 EMSA Kit

GS-0079
535
PIT1

PIT1 EMSA Kit

GS-0068
535
Pit

Pit EMSA Kit

GS-0036
535
Pbx1

Pbx1 EMSA Kit

GS-0035
535
Pax8

Pax8 EMSA Kit

GS-0070
535
Pax3

Pax3 EMSA Kit

GS-0069
535
Pax2

Pax2 EMSA Kit

GS-0067
535
Pax-5

Pax-5 EMSA Kit

GS-0034
535
p53

p53 EMSA Kit

GS-0033
535
OCT4

OCT4 EMSA Kit

GS-0032
535
Oct-1

Oct-1 EMSA Kit

GS-0088
535
NRF2(ARE)

NRF2(ARE) EMSA Kit

GS-0031
535
NRF1

NRF1 EMSA Kit

GS-0091
535
Nkx3-2

Nkx3-2 EMSA Kit

GS-0066
535
Nkx2-5

Nkx2-5 EMSA Kit

GS-0065
535
NFkB

NFkB EMSA Kit

GS-0030
535
NFAT

NFAT EMSA Kit

GS-0028
535
NF-E2

NF-E2 EMSA Kit

GS-0029
535
NF-1

NF-1 EMSA Kit

GS-0027
535
MZF

MZF EMSA Kit

GS-0086
535
MyoD

MyoD EMSA Kit

GS-0064
535
Myc-Max

Myc-Max EMSA Kit

GS-0026
535
Myb

Myb EMSA Kit

GS-0025
535
MEF2

MEF2 EMSA Kit

GS-0024
535
KLF4

KLF4 EMSA Kit

GS-0090
535
IRF

IRF EMSA Kit

GS-0023
535
HSF

HSF EMSA Kit

GS-0054
535
HOXA-5

HOXA-5 EMSA Kit

GS-0061
535
HOX4C

HOX4C EMSA Kit

GS-0085
535
HNF-4

HNF-4 EMSA Kit

GS-0022
535
HNF-1

HNF-1 EMSA Kit

GS-0093
535
HIF

HIF EMSA Kit

GS-0021
535
HEN(NSCL-1)

HEN(NSCL-1) EMSA Kit

GS-0094
535
GR/PR

GR/PR EMSA Kit

GS-0019
535
Gli

Gli EMSA Kit

GS-0072
535
Gfi-1

Gfi-1 EMSA Kit

GS-0077
535
GATA

GATA EMSA Kit

GS-0018
535
GAS/ISRE

GAS/ISRE EMSA Kit

GS-0017
535
FREAC-2 (FOXF2)

FREAC-2 (FOXF2) EMSA Kit

GS-0095
535
FOXO1 (FKHR)

FOXO1 (FKHR) EMSA Kit

GS-0075
535
FOXG1

FOXG1 EMSA Kit

GS-0076
535
FOXD3

FOXD3 EMSA Kit

GS-0055
535
FoxC1

FoxC1 EMSA Kit

GS-0062
535
FOXA1

FOXA1 EMSA Kit

GS-0063
535
FAST-1(FOXH1)

FAST-1(FOXH1) EMSA Kit

GS-0016
535
Ets

Ets EMSA Kit

GS-0015
535
ER

ER EMSA Kit

GS-0014
535
No Probe

EMSA Kits without probes

GS-0000
508
ELK

ELK EMSA Kit

GS-0013
535
EGR

EGR EMSA Kit

GS-0012
535
E2F-1

E2F-1 EMSA Kit

GS-0011
535
CREB

CREB EMSA Kit

GS-0010
535
COUP-TF

COUP-TF EMSA Kit

GS-0084
535
Combo

Combination EMSA Kit with Four Probes

GS-1001
722
CDP

CDP EMSA Kit

GS-0009
535
CBF

CBF EMSA Kit

GS-0008
535
CAR

CAR EMSA Kit

GS-0007
535
C/EBP

C/EBP EMSA Kit

GS-0006
535
Brn-3

Brn-3 EMSA Kit

GS-0005
535
ATF2

ATF2 EMSA Kit

GS-0004
535
AR

AR EMSA Kit

GS-0003
535
AP4

AP4 EMSA Kit

GS-0083
535
AP3

AP3 EMSA Kit

GS-0082
535
AP2

AP2 EMSA Kit

GS-0002
535
AP1

AP1 EMSA Kit

GS-0001
535
AHR

AHR EMSA Kit

GS-0096
535

Specifications

EMSA

SAMPLE TYPE

requires nuclear extract as the starting material, which can either be purchased with the kit or separately. The nuclear extract, catalog number SK-0001, is suitable for both mammalian tissue and cultured cells. 

# OF SAMPLES

Able to run up to 30 samples.

CUSTOMIZATION

We offer the flexibility to synthesize a specific transcription factor if the one you need is not available in our standard kit. Let us know your requirements, and we can tailor the assay to suit your research needs. 

Benefits

No Isotope Required

Safe method of detection using chemiluminescence.

No Probe Preparation

Pre-labeled biotin probes are included in each kit.

Highly Sensitive

The sensitivity of the kit sensitivity is comparable to that of radioactive-based detection methods.

Simple Procedures

Streamlined protocol with ready to use materials and reagents simplify the execution of the experiment.

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