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Cytokine ELISA Kits
Signosis’ Cytokine ELISA Kits offer a streamlined and reliable solution for analyzing individual cytokines. Each kit includes a user-friendly 12x8-well strip plate design, allowing flexible sample throughput, along with a complementary protein standard for accurate quantification.
Principle
In this assay, cytokines present in the sample bind to specific capture antibodies pre-coated on a 96-well plate. After unbound components are washed away, biotin-labeled detection antibodies are added to bind the captured cytokines. Streptavidin-HRP is then introduced, forming a complex with the biotin-labeled antibodies.
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Colorimetric kits: TMB substrate is added, producing a blue color in the presence of HRP. The reaction is stopped with Stop Solution, turning the color yellow. The intensity of the color is measured at an absorbance of 450 nm and is directly proportional to the amount of cytokine present.
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Chemiluminescent kits: A luminescent HRP substrate is added, and the resulting signal is measured as relative light units (RLUs) using a plate luminometer. The luminescent intensity reflects the concentration of each cytokine.
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Benefits
Cost-Effective Analysis
High quality results at a reasonable price.
Efficient & Flexible
Multiple samples can be analyzed simultaneously in a 8x12 strip plate format.
Specific & Simple Procedure
A pair of highly selective antibodies against a specific cytokine. All in one system with a pre-coated 96-well plate.
Compatibility
The assay is suitable for any biological liquid material such as cell lysate, sera, cerebrospinal fluid (CSF), urine, and tissue homogenate.
