Sample type: Adherent cells, Suspension cells
Species reactivity: Mammalian
Assay time: 30-60m
Kit size-: 100, 500 Tests
Storage Conditions: 2-4°C
Cell Viability/Cytotoxicity (CVC) Reagent is a colorimetric WST-8 based assay that provides a simple and accurate method to measure cell proliferation. This is a tetrazolium reduction assays based on the cleavage of the tetrazolium salt WST-8 to water-soluble, orange formazan dye by cellular mitochondrial dehydrogenases (Fig.1). Intensity of dye is a direct proportion of increased activity of the mitochondrial dehydrogenases and therefore number of viable cells which can be quantified by measuring the absorbance at 450 nm.
Ready to use CVC Reagent allow for rapid, sensitive and reliable method for measuring cell viability/cytotoxicity directly from a 96-well plate without any additional processing.
Figure 1. WST-8 Assay Mechanism