Product Name Catalog # Price   Qty
TF Activation Profiling Plate Array I FA-1001 $529
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Characterization

Description:

Transcription factors (TFs) are a group of cellular proteins that play essential roles in regulating gene expression. They act as sensors to monitor cellular change and convert the signals into altered gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under control of multiple TFs. Hence, monitoring the activation of multiple TFs simultaneously is critical to understand the molecular mechanism of cellular regulation underlying cell signaling and gene expression. Signosis’ TF activation profiling plate array I is used for monitoring 48 different TFs including NFkB, AP1, HIF1, and p53.


Applicable Grid:

List of Applicable TFs:

  1 2 3

4

5

6

7 8 9 10

11

12
A AP1 CDP GATA

NF-1

Pit

Stat3

AP1 CDP GATA

NF-1

Pit

Stat3
B AP2 CREB GR/PR NFAT PPAR Stat4 AP2 CREB GR/PR NFAT PPAR Stat4
C AR E2F-1

HIF

NF-E2 PXR Stat5 AR E2F-1

HIF

NF-E2 PXR Stat5
D ATF2 EGR HNF4 NFkB SMAD Stat6 ATF2 EGR HNF4 NFkB SMAD Stat6
E Brn-3 ER IRF OCT4 Sp1 TCF/LEF Brn-3 ER IRF OCT4 Sp1 TCF/LEF
F C\EBP Ets MEF2 p53 SRF YY1 C\EBP Ets MEF2 p53 SRF YY1
G CAR FAST-1 Myb Pax-5 SATB1 TR CAR FAST-1 Myb Pax-5

SATB1

TR
H CBF GAS/ISRE Myc-Max Pbx1 Stat1 TFIID CBF GAS/ISRE Myc-Max Pbx1 Stat1 TFIID

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.

 

 

Data

 



TF activation profiling plate array I analysis the activities of 48 TFs. HeLa cells were treated with and without PMA. The nuclear extracts were prepared and subjected to TF activation profiling plate array.

Literature

View user manual

Citations

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