TCF/LEF EMSA (Electrophoretic-Mobility Shift Assay) kit is used for monitoring TCF/LEF (T cell factor / Lymphoid enhancer factor) DNA binding activities and activation. Upon activation, TCF/LEF can recognize and interact with the unique DNA binding sequence and form protein/DNA complex, which can be separated from free DNA due to the differences in their electrophoretic mobility in native (non-denaturing) polyacrylamide gels.