|Product Name||Catalog #||Price||Qty|
|T cell activation TF Activation Profiling Plate Array with Nuclear Extraction Kit||FA-1015-NE||$729|
T lymphocytes, which are central players in immune responses, consist of several subtypes with different functions. Naive T cells differentiate into effector cells upon encountering antigens. There are several key points during T cell development, which are regulated by a combination of transcription factors. Signosis has developed T cell Activation Transcription Factor Activation Plate Array to monitoring the activities of 8 T cell activation-related TFs simultaneously in mammalian samples, including AP1, E2F1, GATA3, Myb, NFAT4, NFkB, Runx, STAT6.
List of Applicable TFs:
Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.