Product Name Catalog # Price   Qty
T cell activation TF Activation Profiling Plate Array with Nuclear Extraction Kit FA-1015-NE $729
- +

Characterization

Description:

T lymphocytes, which are central players in immune responses, consist of several subtypes with different functions. Naive T cells differentiate into effector cells upon encountering antigens. There are several key points during T cell development, which are regulated by a combination of transcription factors. Signosis has developed T cell Activation Transcription Factor Activation Plate Array to monitoring the activities of 8 T cell activation-related TFs simultaneously in mammalian samples, including AP1, E2F1, GATA3, Myb, NFAT4, NFkB, Runx, STAT6.


Applicable Grid:

List of Applicable TFs:

  1 2 3 4 5 6 7 8 9 10 11 12
A AP1 AP1 AP1 AP1 AP1 AP1 AP1 AP1 AP1 AP1 AP1 AP1
B E2F1 E2F1 E2F1 E2F1 E2F1 E2F1 E2F1 E2F1 E2F1 E2F1 E2F1 E2F1
C GATA3 GATA3 GATA3 GATA3 GATA3 GATA3 GATA3 GATA3 GATA3 GATA3 GATA3 GATA3
D Myb Myb Myb Myb Myb Myb Myb Myb Myb Myb Myb Myb
E NFAT NFAT NFAT NFAT NFAT NFAT NFAT NFAT NFAT NFAT NFAT NFAT
F NFkB NFkB NFkB NFkB NFkB NFkB NFkB NFkB NFkB NFkB NFkB NFkB
G RUNX RUNX RUNX RUNX RUNX RUNX RUNX RUNX RUNX RUNX RUNX RUNX
H STAT6 STAT6 STAT6 STAT6 STAT6 STAT6 STAT6 STAT6 STAT6 STAT6 STAT6 STAT6

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.