|Product Name||Catalog #||Price||Qty|
|Real-Time PCR Assay for Monitoring NFkB Regulated Genes||CL-1001||$479|
NFkB is a ubiquitous transcription factor that plays a key role in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. When it is activated, NFkB mediates the expression of genes involved in immune and inflammatory responses, cell growth control, and apoptosis. To facilitate the monitoring of gene expression representing these biological functions with a limited number of cells, Signosis developed a real-time PCR assay to analyze 8 genes in total RNA or directly in cell lysates.
- All-in-One - All reagents for the experiment, from cell lysis, reverse transcription to PCR assay, are included in one kit.
- No need for RNA preparation - Cell lysates can be used directly in reverse transcription.
- Robust - Full length cDNA can be obtained with the kit.
- Real-time - Analysis can be monitored in real time.
Signosis’ Real-Time PCR assay is to monitor the expression of 8 genes through reverse transcription of mRNA into cDNA and real-time PCR. The purified total RNA or cell lysate is first reverse transcribed into cDNA. Targeted genes are then analyzed with real-time PCR.
Figure 1: Real-Time PCR Analysis of NFkB Target Genes in Cell Lysate and Total RNA. The HeLa cells were treated with TNFa for 6 hours. The cell lysate from 1000 cells and 0.1 ug of total RNA were subjected to the assay. The real-time PCR was conducted on ABI7700.
LiteratureView user manual
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