Product Name Catalog # Price   Qty
Rat P450 cDNA plate Array AP-1181 $479
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Characterization

Description:

The cytochrome P450 family of enzymes play central roles in the oxidative metabolism of a wide range of xenobiotics, including chemicals, sterols, fatty acids and anticancer drugs.  Human CYP genes encode fifty-seven P450 proteins with numerous homolog variants in different tissues. They are highly differentially expressed in response to a variety of environmental chemicals and pharmaceuticals. 

Signosis has developed a plate-based array for profiling 20+ P450-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.

 

 


Applicable Grid:

List of Applicable Genes

  1 2 3 4 5 6 7 8 9 10 11 12
A CYP1a1 Cyp2d22 CYP2s1 CYP1a1 Cyp2d22 CYP2s1 CYP1a1 Cyp2d22 CYP2s1 CYP1a1 Cyp2d22 CYP2s1
B Cyp1a2 Cyp2d26 Cyp3a13 Cyp1a2 Cyp2d26 Cyp3a13 Cyp1a2 Cyp2d26 Cyp3a13 Cyp1a2 Cyp2d26 Cyp3a13
C Cyp1b1 Cyp2d40 Cyp3a16 Cyp1b1 Cyp2d40 Cyp3a16 Cyp1b1 Cyp2d40 Cyp3a16 Cyp1b1 Cyp2d40 Cyp3a16
D Cyp2a12 Cyp2d9 Cyp3a41a Cyp2a12 Cyp2d9 Cyp3a41a Cyp2a12 Cyp2d9 Cyp3a41a Cyp2a12 Cyp2d9 Cyp3a41a
E CYP2a22 CYP2d9 Cyp4a32 CYP2a22 CYP2d9 Cyp4a32 CYP2a22 CYP2d9 Cyp4a32 CYP2a22 CYP2d9 Cyp4a32
F Cyp2b10 Cyp2e1 Cyp4b1 Cyp2b10 Cyp2e1 Cyp4b1 Cyp2b10 Cyp2e1 Cyp4b1 Cyp2b10 Cyp2e1 Cyp4b1
G Cyp2b13 Cyp2f2 18S-rRNA Cyp2b13 Cyp2f2 18S-rRNA Cyp2b13 Cyp2f2 18S-rRNA Cyp2b13 Cyp2f2 18S-rRNA
H Cyp2d10 Cyp2j6 Blank Cyp2d10 Cyp2j6 Blank Cyp2d10 Cyp2j6 Blank Cyp2d10 Cyp2j6 Blank

Principle

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.

Data



Analysis of expression of P450 genes with P450 cDNA Plate Array.  2ug of RNAs from rat brain was subjected to P450 cDNA plate assay. The cDNA plate array were detected with HRP by a luminometer plate reader.

Literature

View user manual

Citations


A novel vascular EET synthase: role of CYP2C7 Sun D, Jiang H, Wu H, Yang Y, Kaley G, Huang A. Am J Physiol Regul Integr Comp Physiol. 2011 Dec;301(6):R1723-30.