Product Name Catalog # Price   Qty
Neurodegenerative Disease TF Activation Profiling Plate Array FA-1013 $629
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Characterization

Description:

Neurodegenerative disease, include Parkinson’s, Alzheimer’s and Huntington’s disease, is a disease that directly affect the neurons in the human brain, which result in progressive degeneration and / or death of nerve cells. This disease is associated with the accumulation of misfolded proteins which affect proteostasis. Multiple transcription factors and target genes have been widely reported to associate with proteostasis, including protein synthesis, folding, trafficking and degradation. Signosis has developed Neurodegenerative Disease Transcription Factor Activation Plate Array to analyze the activities of 15 Neurodegenerative Disease-related TFs simultaneously in mammalian samples, including AP1, CREB, ER, FOXO, FOXA1, GATA, HSF1, NFKB, NRF2, NRRR1, PITX3, PPAR, SP1, TBP, XBP1.


Applicable Grid:

 List of Applicable TFs:

  1 2 3 4 5 6 7 8 9 10 11 12
A AP1 NRF2 AP1 NRF2 AP1   NRF2 AP1   NRF2 AP1 NRF2 AP1 NRF2 
B CREB NURR1 CREB NURR1 CREB   NURR1 CREB   NURR1    CREB   NURR1  CREB   NURR1 
C ER PITX1 ER PITX1 ER  PITX1  ER    PITX1 ER   PITX1  ER   PITX1 
D FOXO1 PPAR FOXO1 PPAR FOXO1   PPAR FOXO1   PPAR  FOXO1   PPAR   FOXO1 PPAR 
E FOXA1 SP1 FOXA1 SP1 FOXA1   SP1 FOXA1  SP1  FOXA1   SP1      FOXA1    SP1 
F GATA TBX GATA TBX GATA  TBX  GATA   TBX    GATA  TBX   GATA   TBX  
G HSF1 XBP-1 HSF1 XBP-1 HSF1  XBP-1   HSF1  XBP-1    HSF1  XBP-1  HSF1  XBP-1 
H NFkB Blank NFkB Blank  NFkB  Blank  NFkB  Blank  NFkB  Blank  NFkB  Blank

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.

 

Literature

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