Product Name Catalog # Price   Qty
Mouse Mix & Match Cytokine ELISA Strip CUS-EA-2001 $499
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Description:

Cytokines are essential molecules play crucial roles in many biological functions, including viral infection, inflammation, immunity, and hematopoiesis. Cytokines are produced by a variety of cell types in response to different stimuli. In addition, the expression of cytokine genes appears to be regulated by complex mechanism. Expression of one cytokine gene could be regulated by other cytokines. Dysregulation of cytokine gene expression may be caused by chromosomal alterations or by infection of viruses that induce activation or inactivation of the expression machinery. Therefore, profiling of these cytokines is critical to understanding these biological functions. Signosis’ Mouse Cytokine ELISA Strip I Profiling Assay allows simultaneously profiling 8 cytokines. Each well of the strip is coated with a primary antibody against a specific cytokine and total 8 wells of a strip target 8 different cytokines. The difference of these proteins between two samples can be determined through data comparison.


Principle

Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.



 

 



 

Citations

 

1. Extracellular signal regulated kinase 5: A potential therapeutic target formalignant mesotheliomas. Shukla A, Miller JM, Cason C, Sayan M, Macpherson MB, Beuschel SL, Hillegass JM, Vacek PM, Pass H,Mossman BT. Clin Cancer Res., 2013 Apr;19(8):2071-83.

2. Carrageenan-induced Colonic Inflammation is Reduced in Bcl10-Null Mice and Increased in IL-10 Deficient Mice. Bhattacharyya S, Xue L, Devkota S, Chang E, Morris S, Tobacman JK. Mediators Inflamm., 2013;2013:397642.

3. Minocycline has anti-inflammatory effects and reduces cytotoxicity in an ex vivo spinal cord slice culture model of West Nile virus infection. Eamon D. Quick, Scott Seitz, Penny Clarke, and Kenneth L. Tyler.  Journal of Virology, June 2017, doi:10.1128/jvi.00569-17.