How to order this kit:


Step 1: choose miRNA plate

Product Name Catalog # Price   Qty
miRNA Real-Time PCR Assay Kit CL-0004 $449
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Step 2: choose oligo for miRNA of interest

Product Name Catalog # Price   Qty
let-7a-5p PO-0001 $70
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let-7b-5p PO-0002 $70
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let-7c-5p PO-0003 $70
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let-7d-5p PO-0004 $70
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let-7e-5p PO-0005 $70
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let-7f-5p PO-0006 $70
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let-7g-5p PO-0007 $70
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let-7i-5p PO-0008 $70
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miR-1 PO-0009 $70
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miR-7-5p PO-0010 $70
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miR-9-5p PO-0011 $70
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miR-10a-5p PO-0012 $70
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miR-15a-5p PO-0013 $70
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miR-16-5p PO-0014 $70
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miR-17-3p PO-0015 $70
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miR-17-5p PO-0016 $70
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miR-18a-5p PO-0017 $70
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miR-19a-3p PO-0018 $70
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miR-20a-5p PO-0019 $70
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miR-21-5p PO-0020 $70
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miR-22-3p PO-0021 $70
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miR-23a-3p PO-0022 $70
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miR-24-3p PO-0023 $70
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miR-25-3p PO-0024 $70
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miR-26a-5p PO-0025 $70
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miR-27a-3p PO-0026 $70
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miR-28-5p PO-0027 $70
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miR-29a-3p PO-0028 $70
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miR-29b-3p PO-0029 $70
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miR-29c-3p PO-0030 $70
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miR-30a-3p PO-0031 $70
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miR-30a-5p PO-0032 $70
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miR-30b-5p PO-0033 $70
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miR-30c-5p PO-0034 $70
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miR-31-5p PO-0035 $70
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miR-92-3p PO-0036 $70
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miR-92b-3p PO-0037 $70
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miR-93-5p PO-0038 $70
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miR-95-3p PO-0039 $70
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miR-96-5p PO-0040 $70
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miR-99a-5p PO-0041 $70
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miR-100-5p PO-0042 $70
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miR-101-3p PO-0043 $70
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miR-103-3p PO-0044 $70
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miR-106a-5p PO-0045 $70
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miR-106b-5p PO-0046 $70
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miR-107 PO-0047 $70
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miR-122a-5p PO-0048 $70
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miR-124a-3p PO-0049 $70
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miR-125a-5p PO-0050 $70
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miR-125b-5p PO-0051 $70
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miR-126-3p PO-0052 $70
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miR-127-5p PO-0053 $70
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miR-128a-3p PO-0054 $70
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miR-128b-3p PO-0055 $70
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miR-131 PO-0056 $70
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miR-132-3p PO-0057 $70
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miR-133a-3p PO-0058 $70
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miR-133b PO-0059 $70
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miR-134-5p PO-0060 $70
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miR-135b-5p PO-0061 $70
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miR-136-5p PO-0062 $70
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miR-137 PO-0063 $70
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miR-140-5p PO-0064 $70
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miR-141-3p PO-0065 $70
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miR-142-3p PO-0066 $70
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miR-142-5p PO-0067 $70
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miR-143-3p PO-0068 $70
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miR-144-3p PO-0069 $70
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miR-145-5p PO-0070 $70
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miR-146a-5p PO-0071 $70
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miR-146b-5p PO-0072 $70
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miR-148a-3p PO-0073 $70
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miR-149-5p PO-0074 $70
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miR-150-5p PO-0075 $70
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miR-151-3p PO-0076 $70
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miR-153-3p PO-0077 $70
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miR-152-3p PO-0078 $70
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miR-154-5p PO-0079 $70
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miR-155-5p PO-0080 $70
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miR-181a-5p PO-0081 $70
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miR-181b-5p PO-0082 $70
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miR-181c-5p PO-0083 $70
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miR-181d-5p PO-0084 $70
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miR-182-5p PO-0085 $70
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miR-183-5p PO-0086 $70
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miR-184 PO-0087 $70
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miR-185-5p PO-0088 $70
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miR-186-5p PO-0089 $70
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miR-188-5p PO-0090 $70
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miR-190-5p PO-0091 $70
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miR-191-5p PO-0092 $70
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miR-192-5p PO-0093 $70
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miR-193a-3p PO-0094 $70
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miR-193a-5p PO-0095 $70
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miR-194-5p PO-0096 $70
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miR-195-5p PO-0097 $70
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miR-196a-5p PO-0098 $70
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miR-196b-5p PO-0099 $70
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miR-197-3p PO-0100 $70
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miR-198 PO-0101 $70
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miR-199a-5p PO-0102 $70
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miR-199a*-5p PO-0103 $70
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miR-199b-5p PO-0104 $70
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miR-200a-3p PO-0105 $70
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miR-200c-3p PO-0106 $70
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miR-202-5p PO-0107 $70
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miR-203a PO-0108 $70
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miR-204-5p PO-0109 $70
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miR-205-5p PO-0110 $70
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miR-206 PO-0111 $70
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miR-210-3p PO-0112 $70
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miR-213 (miR-181a-3p) PO-0113 $70
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miR-214-3p PO-0114 $70
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miR-215-5p PO-0115 $70
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miR-216-5p PO-0116 $70
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miR-218-5p PO-0117 $70
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miR-219 PO-0118 $70
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miR-221-3p PO-0119 $70
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miR-222-3p PO-0120 $70
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miR-223-3p PO-0121 $70
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miR-224-5p PO-0122 $70
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miR-296-5p PO-0123 $70
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miR-329 PO-0124 $70
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miR-337-3p PO-0125 $70
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miR-338 PO-0126 $70
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miR-342-3p PO-0127 $70
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miR-345-5p PO-0128 $70
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mir-376c-3p (miR-368) PO-0129 $70
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miR-369-3p PO-0130 $70
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miR-369-5p PO-0131 $70
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miR-371a-3p PO-0132 $70
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miR-372-3p PO-0133 $70
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miR-373-5p PO-0134 $70
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miR-375 PO-0135 $70
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miR-488-5p PO-0136 $70
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miR-497-5p PO-0137 $70
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miR-499-5p PO-0138 $70
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miR-507 PO-0139 $70
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miR-609 PO-0140 $70
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miR-720 PO-0141 $70
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miR-760 PO-0142 $70
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U6 PO-0512 $70
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sno234 PO-0510 $70
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RNU48 PO-0511 $70
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Description:

miRNAs are known to involve in multiple biological functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer biomarkers. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.

 


Principle

In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.

Data


Comparison of miRNA detection in HeLa RNA and Cell lysate. HeLa total RNA was prepared with Trizol reagent. HeLa cell lysate from 200 cells was prepared with Cell Lysis Buffer. The samples were subjected to miRNA real time PCR assay on a 7700 system.

Literature

View user manual

Citations

1.Molecular basis of cardiac endothelial-to mesenchymal  transition(EndMT): differential expression of microRNAs during EndMT. Ghosh AK, Nagpal V, Covington JW, Michaels MA, Vaughan DE. Cell Signal. 2012 May;24(5):1031-6.
 
2.Non-Coding RNAs: Functional Aspects and Diagnostic Utility in Oncology. Kim T, Reitmair A. Int J Mol Sci., 2013 Mar;14(3):4934-68.
 
3.Recent Advance in Biosensors for microRNAs Detection in Cancer Silvia Catuogno, Carla L. Esposito,  Cristina Quintavalle, Laura Cerchia, Gerolama Condorelli  and Vittorio de Franciscis 1, Cancers 2011, 3, 1877-1898; doi:10.3390/cancers3021877.
 
4.Circulating Blood MicroRNAs In The Diagnosis Of Lung Cancer. Le W, Wang W, Ghani M, Upadhyay D. Am. J. Respir. Crit. Care Med., May 2010; 181: A3482.
 
5.MicroRNA-17-5p post-transcriptionally regulates p21 expression in irradiated betel quid chewing-related oral squamous cell carcinoma cells. Wu SY, Lin KC, Chiou JF, Jeng SC, Cheng WH, Chang CL, Lin WC, Wu LL, Lee HL, Chen RJ. Strahlenther Onkol., 2013 Jun[Epub ahead of print].