Product Name Catalog # Price   Qty
Insulin Resistant TF Activation Profiling Plate Array FA-1014 $629
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Characterization

Description:

Insulin resistance and central adiposity are strong risk indicators for type 2 diabetes and coronary heart disease. Insulin resistance is a pathological condition in which cells fail to respond normally to the hormone insulin, leading to high blood sugar. Many transcription factors are related to the regulation of adipocyte metabolism, by helping glucose uptake and lipid accumulation, meanwhile reducing insulin sensitivity. Signosis has developed Insulin Resistant Transcription Factor Activation Plate Array to analyze the activities of 16 Insulin Resistant-related TFs simultaneously in mammalian samples, including AP2, CREB, EGR-1, FOXO1, HNF-1, HSF-1, LXR, MAFA, NEUROD, NKX2.2, NKX6.1, PAX4, PAX6, PDX-1, PPAR, SREBP1.


Applicable Grid:

 List of Applicable TFs:

  1 2 3 4 5 6 7 8 9 10 11 12
A AP2 NEUROD AP2 NEUROD AP2 NEUROD AP2 NEUROD AP2 NEUROD AP2 NEUROD
B CREB NKX2.2 CREB NKX2.2 CREB NKX2.2 CREB NKX2.2 CREB NKX2.2 CREB NKX2.2
C EGR-1 NKX6.1 EGR-1 NKX6.1 EGR-1 NKX6.1 EGR-1 NKX6.1 EGR-1 NKX6.1 EGR-1 NKX6.1
D FOXO1 PAX4 FOXO1 PAX4 FOXO1 PAX4 FOXO1 PAX4 FOXO1 PAX4 FOXO1 PAX4
E HNF-1 PAX6 HNF-1 PAX6 HNF-1 PAX6 HNF-1 PAX6 HNF-1 PAX6 HNF-1 PAX6
F HSF-1 PDX1 HSF-1 PDX HSF-1 PDX HSF-1 PDX HSF-1 PDX HSF-1 PDX
G LXR PPAR LXR PPAR LXR PPAR LXR PPAR LXR PPAR LXR PPAR
H MAFA SREBP1 MAFA SREBP1 MAFA SREBP1 MAFA SREBP1 MAFA SREBP-1 MAFA SREBP-1

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.

 

Literature

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