The Wnt signaling pathway is important to both embryonic development and tumorigenesis. ß-Catenin, the central component of the pathway, functions as a co-factor of the TCF/LEF family of transcription factors. Together, they activate transcription of Wnt target genes by binding to the promoters of downstream target genes involved in cell proliferation, survival, and migration. Signosis’ Wnt/ß-Catenin signaling pathway cDNA plate array can simultaneously measure the expression of 20+ genes that participate in the pathway and can compare the differences in the expression of these genes in different samples.
Signosis has developed a plate-based array for profiling 20+ Wnt-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
List of Applicable Genes
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
Human Wnt cDNA plate array analysis. HeLa cells were treated with or without 2uM LPA for 8 hours. Cells were collected for profiling 23 genes with the cDNA plate array assay.
LiteratureView user manual
1. Expression of Wnt3 activates Wnt/β-catenin pathway and promotes EMT-like phenotype intrastuzumab resistant HER2-overexpressing breast cancer cells. Vadgama JV, Wu Y, Ginther C, Kim J, Mosher N, Chung S, Slamon D. Mol Cancer Res. 2012 Oct 15.
2. A83-01 inhibits TGF-β-induced upregulation of Wnt3 and epithelial to mesenchymal transition in HER2-overexpressing breast cancer cells. Yanyuan Wu, Trinh Tran, Sami Dwabe, Marianna Sarkissyan, Juri Kim, Miguel Nava, Sheilah Clayton, Richard Pietras, Robin Farias-Eisner, Jaydutt V. Vadgama. Breast Cancer Research and Treatment. 2017 March 23. doi: 10.1007/s10549-017-4211-y.