Product Name Catalog # Price   Qty
Human Toxicity cDNA Plate Array AP-0203 $479
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Characterization

Description:

Drug toxicity mainly results as a byproduct of drug metabolism, in which a drug may be biotransformed by drug metabolizing enzymes to toxic or nontoxic metabolites. A number of protein families such as P450s, UDP-glucuronosyltransferases, sulfotransferases, organic-anion transporters and multidrug resistance proteins are involved in drug metabolizing processes. Changes in the expression level of these enzymes are a key factor responsible for individual variation in drug metabolism. 

Signosis has developed a plate-based array for profiling 20+ Toxicity-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.

 


Applicable Grid:

List of Applicable Genes

  1 2 3 4 5 6 7 8 9 10 11 12
A ABCB11 CYP2C8 MDR1 ABCB11 CYP2C8 MDR1 ABCB11 CYP2C8 MDR1 ABCB11 CYP2C8 MDR1
B Beta-Actin CYP2C9 OATP-C Beta-Actin CYP2C9 OATP-C Beta-Actin CYP2C9 OATP-C Beta-Actin CYP2C9 OATP-C
C CYP-1a1 CYP2E1 UGT1A27 CYP-1a1 CYP2E1 UGT1A27 CYP-1a1 CYP2E1 UGT1A27 CYP-1a1 CYP2E1 UGT1A27
D CYP-1a2 CYP3A4 UGT1A28 CYP-1a2 CYP3A4 UGT1A28 CYP-1a2 CYP3A4 UGT1A28 CYP-1a2 CYP3A4 UGT1A28
E CYP1B1 CYP3A5/A7 UGT1A6 CYP1B1 CYP3A5/A7 UGT1A6 CYP1B1 CYP3A5/A7 UGT1A6 CYP1B1 CYP3A5/A7 UGT1A6
F CYP-2A6 GAPDH UGT1A8 CYP-2A6 GAPDH UGT1A8 CYP-2A6 GAPDH UGT1A8 CYP-2A6 GAPDH UGT1A8
G CYP-2B6 CYP4A11 UGT1A9 CYP-2B6 CYP4A11 UGT1A9 CYP-2B6 CYP4A11 UGT1A9 CYP-2B6 CYP4A11 UGT1A9
H CYP2C19 CYP4B1 Blank CYP2C19 CYP4B1 Blank CYP2C19 CYP4B1 Blank CYP2C19 CYP4B1 Blank

Principle

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.

Literature

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