Product Name Catalog # Price   Qty
Human T Cell Receptor Signaling cDNA Plate Array AP-0191 $479
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Characterization

Description:

T-Cells are a subset of lymphocytes that play a key role in the immune response. T-Cell Receptor (TCR) is a complex of integral membrane proteins that participates in the activation of T-Cells in response to antigen. This activation involves subunits of the receptor complex as well as other membrane-associated molecules that couple the extracellular liganding event to downstream signaling pathways such as the increase of inositol phosphates, the elevation of intracellular calcium levels and protein phosphorylation. TCR activation promotes a number of signaling cascades that ultimately determine cell fate through regulating cytokine production, cell survival, proliferation, and differentiation. 

Signosis has developed a plate-based array for profiling 20+ Human T Cell-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
 


Applicable Grid:

List of Applicable Genes

  1 2 3 4 5 6 7 8 9 10 11 12
A CD45 JNK PLCr CD45 JNK PLCr CD45 JNK PLCr CD45 JNK PLCr
B ELK JUN Raf1 ELK JUN Raf1 ELK JUN Raf1 ELK JUN Raf1
C B-actin LAT Ras B-actin LAT Ras B-actin LAT Ras B-actin LAT Ras
D GADS LCK SLP76 GADS LCK SLP76 GADS LCK SLP76 GADS LCK SLP76
E GRB2 NFAT SOS GRB2 NFAT SOS GRB2 NFAT SOS GRB2 NFAT SOS
F IL2 NFkB TCRz IL2 NFkB TCRz IL2 NFkB TCRz IL2 NFkB TCRz
G IP3R p38 Zap70 IP3R p38 Zap70 IP3R p38 Zap70 IP3R p38 Zap70
H ITK PKC-theta blank ITK PKC-theta blank ITK PKC-theta blank ITK PKC-theta blank

Principle

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.

Literature

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