Stem cells have important characteristics distinguishing from other cell types. They are unspecialized cells capable of renewing themselves through cell division and differentiating into a variety of cell lineages. In addition, they serve as an internal repair system, replenishing specialized cells, in order to maintain the normal turnover of regenerative organs. Identification of a set of stem cell–specific genes can function as stem cell molecular biomarkers to facilitate stem cell research.
Signosis has developed a plate-based array for profiling 20+ Stem Cell-related genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
List of Applicable Genes
|D||VE-Cadherin||Integrin b1||Beta-actin||TAZ||VE-Cadherin||Integrin b1||Beta-actin||TAZ||VE-Cadherin||Integrin b1||Beta-actin||TAZ|
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
LiteratureView user manual
Stem cells from foreign body granulation tissue accelerate recovery from acute kidney injury. Patel J, Pancholi N, Gudehithlu KP, Sethupathi P, Hart PD, Dunea G, Arruda JA, Singh AK. Nephrol Dial Transplant. 2012 May;27(5):1780-6.