Apoptosis is a cellular process involving cytoplasmic condensation and cell shrinkage, chromatin condensation, membrane blebbing, nuclear fragmentation, and the formation of apoptotic bodies, which leads to programmed cell death. During the process, a series of biochemical reactions occur, such as the activation of intracellular proteases and regulation of apoptosis-associated genes. Signosis’ Apoptosis-Associated cDNA Plate Array provides an approach for profiling the expression of apoptosis-regulating genes.
Signosis has developed a plate-based array for profiling 20+ Apoptosis-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
List of Applicable Genes
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
Apoptosis cDNA array analysis of gene expression in HeLa. HeLa cell were treated with and without 2uM camptothcin for 16 hours. Total RNAs were prepared for the cDNA plate array assay.
LiteratureView user manual
Endogenous Human CaMKII Inhibitory Protein Suppresses Tumor Growth by Inducing Cell Cycle Arrest and Apoptosis through Down-regulation of the Phosphatidylinositide 3-Kinase/Akt/HDM2 Pathway. Ma S, Yang Y, Wang C, Hui N, Gu L, Zhong H, Cai Z, Wang Q, Zhang Q, Li N, Cao X. J Biol Chem. 2009 Sep 11;284(37):24773-82.