|Product Name||Catalog #||Price||Qty|
|HIF Luciferase Reporter Hela Stable Cell Line||SL-0023-FP||$2,000|
HIF Responsive Luciferase Reporter Hela Stable Cell Line is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia Response Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Hypoxia-Inducible Factor (HIF)-1 is a dimeric protein complex that plays a central role in the response to low oxygen concentrations, or hypoxia, and is a crucial physiological regulator of homeostasis, vascularization, and anaerobic metabolism. HIF is a transcription factor regulating gene expression by binding to their DNA recognition site on the target genes. Furthermore, HIF-1 has been widely studied because of its perceived therapeutic potential. HIF-1 allows survival and proliferation of cancerous cells due to its angiogenic properties, and the inhibition potentially could prevent the spread of cancer. With a growing understanding of the HIF-1 pathway, it has become an attractive goal to analyze the inhibition and stimulation of HIF transcriptional activity via small molecules.
Signosis has developed HIF Luciferase Reporter Stable Cell Line by transfection of HIF luciferase reporter vector along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for CoCl2 or deferroxamine-induced luciferase activity.
Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation.
Analysis of SL-0023 HIF reporter activity in response to DFO treatment. The Hela cells were seeded on a 96-well plate for overnight with DMEM including 10% FBS. The cells then were treated with or without varying dose of deferoxamine (DFO) in DMEM and 10% FBS for 18 hours. Hela-HIF Luciferase Reporter Cell Line exhibits dose-dependent increase in luciferase activity when compared to untreated cells.