Product Name Catalog # Price   Qty
EGFR (E19del) Stably Expressing HCC827 Cell Line EL-005-FP $3,000
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Description:

Epidermal growth factor receptor (EGFR) is a cell-surface receptor with intrinsic intracellular protein-tyrosine kinase (TK) activity. Ligand binding induces EGFR dimerization and phosphorylation, leading to the activation of EGFR signaling pathway. In several malignancies such as non-small cell lung cancer (NSCLC), EGFR signaling is deregulated due to mutations in EGFR, which results in uncontrolled proliferation and migration of tumor cells. EGFR mutations can lead to “oncogene-addicted” cancers, where the tumor cells depend on the mutated EGFR for cell survival and malignant phenotype.  One of the most common EGFR mutations found in human patients is L858R substitution in exon 21, within the activation loop of EGFR. Patients with this mutation are sensitive to EGFR tyrosine kinase inhibitors (TKIs) such as gefitinib or erlotinib, whereas patients with wild type EGFR are not sensitive to TKI. Another clinically relevant mutation associated with acquired gefitinib and erlotinib resistance is T790M, found in exon 20. Cells expressing EGFR with both L858R and T790M mutations are resistant to induced apoptosis in the presence of gefitinib or erlotinib. 

 

We offer EL-005, an HCC827 stable cell line expressing EGFR (E19del). The EGFR exon 19 mutation is an 11-18 bp in-frame deletion around the ATP-binding site encoded in exon 19 of the EGFR gene, observed in ~15-20% of lung cancer patients. This cell line can be used to study the molecular mechanism underlying susceptibility of tumors to the drugs (i.e. gefitinib and erlotinib) as well as screening and validating new TKIs. 

 


Data

Dose-dependent growth inhibition of HCC827 cells harboring EGFR E19del (EL-005), control HCC827 cell line (EL-007) and representative Erlotinib resistant cell line, EGFR E19del+T790M (EL-006). The cells were treated with the indicated dose of erlotinib for 72 hours and cell viability was measured using Signosis CVC reagent. EGFR E19del mutation stably expressing HCC827 cells (EL-005) were more sensitive to TKI erlotinib than its resistance derivative EGFR E19del+T790M stably expressing HCC827 cells (EL-006).

 

Literature

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