Signosis Luciferase Substrate

Benefits (Substrate):
  • High signal and responsive - Produces strong and stable light signal.
  • Economical - Less than $0.40 per reaction.
  • Compatible - Works with all Signosis luciferase products and third-party firefly luciferase systems.
Benefits (Buffer):
  • Easy to use
Product Name Catalog # Price   Qty
Firefly Luciferase Lysis Buffer 5X (10mL) LS-001 $65
- +
Firefly Luciferase Substrate (15mL) LUC015 $150
- +
Firefly Luciferase Substrate (100mL) LUC100 $499
- +

Cell Viability Cytotoxicity (CVC) Reagent

Sample type: Adherent cells, Suspension cells

Species reactivity: Mammalian

Assay time: 30-60m

Kit size-: 100, 500 Tests

Storage Conditions: 2-4°C

 

Overview

Cell Viability/Cytotoxicity (CVC) Reagent is a colorimetric WST-8 based assay that provides a simple and accurate method to measure cell proliferation. This is a tetrazolium reduction assays based on the cleavage of the tetrazolium salt WST-8 to water-soluble, orange formazan dye by cellular mitochondrial dehydrogenases (Fig.1). Intensity of dye is a direct proportion of increased activity of the mitochondrial dehydrogenases and therefore number of viable cells which can be quantified by measuring the absorbance at 450 nm.

Ready to use CVC Reagent allow for rapid, sensitive and reliable method for measuring cell viability/cytotoxicity directly from a 96-well plate without any additional processing.

 


 Figure 1.  WST-8 Assay Mechanism

 

Product Name Catalog # Price   Qty
Cell Viability Cytotoxicity (CVC) Reagent 1ml CVC001 $48
- +
Cell Viability Cytotoxicity (CVC) Reagent 5ml CVC005 $115
- +

Components

List of buffers and other components for miRNA assays, ELISA, PCR, and TF assays:

 

Product Name Catalog # Price   Qty
Detection Sheets (GS-00XX) GSDS001 $12
- +
Annealing Buffer (AP-000X, CL-0004, CL-0005) CAB001 $55
- +
5X Assay Wash Buffer (EA-XXXX) AWB001 $35
- +
Bead Wash Buffer (CL-0004, CL-0005, CL-0006) CBW001 $95
- +
Beads Binding Buffer (CL-0004, CL-0005, CL-0006) CBB001 $65
- +
Blocking Buffer (FA-100X, GS-00XX, NB-0001, NB-1001) CBB101 $65
- +
Cancer MiRNA Array Membrane (AP-0003) CAM003 $95
- +
Cancer MiRNA Array Refil Kit (AP-0003) AP1003 $420
- +
Cancer Mix III (AP-0003) COM003 $75
- +
15% Denature Gel (NB-0001, NB-1001) NBDG01 $55
- +
5X Detection Wash Buffer (FA-100X) CDW001 $35
- +
1X Diluent Buffer (EA-XXXX) DB001 $35
- +
Elution Buffer (FA-100X) EB001 $35
- +
Filter Binding Buffer (FA-100X) FBB001 $38
- +
Filter Wash Buffer (FA-100X) FWB001 $35
- +
Human MiRNA Array I Membrane (AP-0001) CAM001 $65
- +
Human MiRNA Array II Membrane (Ap-0002) CAM002 $75
- +
Human MiRNA Array I Refil Kit (AP-0001) AP1001 $300
- +
Human MiRNA Array II Refil Kit (AP-0002) AP1002 $320
- +
1X Hybridization Buffer for cDNA Plate Assay (AP-XXXX) CHB01 $35
- +
5X Hybridization Wash Buffer for cDNA Plate Assay (AP-XXXX) CHW001 $35
- +
Labeling Mix (AP-000X) CLM001 $75
- +
Ligation Buffer (AP-000X) LB001 $75
- +
miRNA qPCR Primer (CL-0004) QPP001 $25
- +
Molecular Markers (20nt & 60nt) (NB-0001, NB-1001) NBMM001 $45
- +
1X NB Hybridization Buffer for NB Assay (NB-0001, NB-1001) NBHB01 $65
- +
5X NB Hyb Wash Buffer for NB Assay (NB-0001, NB-1001) NBHW01 $55
- +
NB Membranes (NB-0001, NB-1001) NBM01 $35
- +
Oligo Mix I (AP-0001) COM001 $55
- +
Oligo Mix II (AP-0002) COM002 $55
- +
Plate Hybridization Buffer (MA-XXXX) PHB001 $38
- +
Stop Solution(2N H2SO4) (EA-XXXX) SS001 $35
- +
Streptavidin Magnetic Beads (AP-000X) CSB001 $45
- +
Streptavidin-HRP Conjugate (EA-XXXX) CSH001 $55
- +
Substrate A for Membranes (GS-00XX, NB-0001, NB-1001) CAS001 $45
- +
Substrate B for Membranes (GS-00XX, NB-0001, NB-1001) CBS001 $45
- +
Substrate A for Plate (FA-100X) SAP001 $45
- +
Substrate B for Plate (FA-100X) SBP001 $45
- +
Substrate Dilution Buffer (FA-100X) SDB002 $35
- +
Substrate for ELISA (EA-XXXX) SUBE001 $35
- +
SYBR Green cDNA PCR Master Mix (CL-0003) SGM002 $130
- +
SYBR Green miRNA PCR Master Mix (CL-0005, CL-0006) SGM001 $130
- +
TF Binding Buffer Mix (FA-100X) TBB001 $35
- +
TF Plate Hybridization Buffer (FA-100X) TPHB001 $35
- +
TF Probe Mix (FA-100X) TPM001 $75
- +