miRNAs are small non-coding RNA molecules that regulate up to 30% of mammalian gene expression. They serve as post-transcriptional regulators and bind to complementary sequences on target messenger RNA transcripts (mRNAs), resulting in translational repression or target degradation. So far, more than 400 miRNAs have been identified in the human genome and many of them are only different in one or a few nucleotides. Dysregulation of miRNA can be associated with many diseases such as cancer; therefore, the research of miRNAs is very important.

Signosis provides a variety of innovative tools for the research of miRNAs including miRNA arrays for profiling multiple miRNAs simultaneously or monitoring one specific miRNAs.

  

miRNA Arrays

The miRNA Arrays are used for simultaneously profiling up to 132 miRNAs.
Benefits:
  • Highly discriminative power - It is able to differentiate all miRNA isoforms. All let7 isoforms can be clearly distinguished in the assay.
  • Linear amplification Captured miRNAs are subjected to T7 amplification without introduction of bias in PCR.
  • No pre-isolation of miRNA - Total RNA can be directly used for the assay without pre-isolation of miRNA.
  • Simple procedure - The assay is simple and straightforward.
Product Name Catalog # Price   Qty
Human miRNA Array I AP-0001 $469
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Human miRNA Array II AP-0002 $549
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Human miRNA Array III AP-0008 $629
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Human miRNA Array IV AP-0009 $549
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Cancer miRNA Array AP-0003 $629
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Apoptosis-Associated miRNA Array AP-0004 $469
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Stem cell-associated miRNA array AP-0007 $549
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Mouse miRNA Array AP-0005 $629
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Rat miRNA Array AP-0006 $629
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miRNA Real Time PCR

With the miRNA Real-Time PCR kits, miRNA can be analyzed from total RNA or from cell lysates directly without cDNA conversion.

Benefits:
  • Convenient All reagents, from cell lysis to PCR, are included in one kit.
  • No RNA preparation Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.
  • Real-time Analysis can be monitored in real time.
Product Name Catalog # Price   Qty
miRNA Real-Time PCR Assay Kit CL-0004 $449
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miR-17-92 Cluster Real-Time PCR Assay Kit CL-0005 $469
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Let7 Isoform Real-Time PCR CL-0006 $469
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miRNA Northern Blot Assays

The assay kits are non-radioactive with all reagents included, and the probes are biotin pre-labeled. Signosis also provides the Highly Sensitive Northern Blot Assay kits, which are 100 times more sensitive than conventional chemiluminescent detection.

Benefits:

  • No Isotope required Safe method of detection using chemiluminescence.
  • No probe preparation Pre-labeled biotin probes included in each kit.
  • All components in one kitEverything needed to perform the assay is included.
  • Simple procedure No miRNA isolation required, total RNA can be used directly.
Product Name Catalog # Price   Qty
miRNA Northern Blot Assay Kit including 2 gels NB-0001 $369
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miRNA Northern Blot Assay Kit excluding 2 gels NB-0002 $339
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Highly sensitive miRNA Northern Blot Assay Kit NB-1001 $389
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miRNA Luciferase Reporter Vectors

miRNAs have been shown to regulate many developmental and physiological processes, and the deregulation of miRNAs has been linked to a number of disease processes, including cancer. miRNAs play important roles in fine-tuning gene expression regulators referred to as “dimmer switches” because of their ability to repress gene expression without completely silencing it.  The precise monitoring of miRNA expression particularly in cell-based assay, can help to reveal the potential miRNA impacts on cancer etiology, differential stages and therapy development.  Signosis developed the miRNA reporter vectors.  Each reporter vector was constructed by inserting miRNA exactly complementary sequence (~20nt) as corresponding miRNA binding sites on the downstream of luciferase reporter gene.  When miRNA in cells binds to the complementary site at 3'UTR downstream of reporter luciferase gene, it leads to repression of the expression of luciferase and results in reduction of luciferase enzyme activity. The miRNA expression level quantitatively corresponds with the luciferase activities.

 

100+ luciferase reporter vectors are available for monitoring different miRNAs in vivo. Signosis also provides custom services for any unlisted miRNA that you might be interested in.

Benefits:
  • Functional assay Monitor miRNA expression in cells.
  • Quantitative analysis - Expression and activation of miRNAs are measured through suppression of firefly luciferase gene expression.
Product Name Catalog # Price   Qty
pLet7b-Luc LR-0001 $369
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pMiR-7-5p-Luc LR-0002 $369
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pMiR-7b-5p-Luc LR-0003 $369
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pMiR-15a-5p-Luc LR-0004 $369
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pMiR-16-5p-Luc LR-0005 $369
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pMiR-17-5p-Luc LR-0006 $369
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pMiR-17-3p-Luc LR-0007 $369
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pMiR-19a-3p-Luc LR-0008 $369
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pMiR-20a-5p-Luc LR-0009 $369
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pMiR-21-5p-Luc LR-0010 $369
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pMiR-24-3p-Luc LR-0011 $369
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pMiR-26a-5p-Luc LR-0012 $369
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pMiR-29a-3p-Luc LR-0013 $369
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pMiR-34a-5p-Luc LR-0014 $369
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pMiR-101-3p-Luc LR-0015 $369
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pMiR-106a-5p-Luc LR-0016 $369
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pMiR-107-Luc LR-0017 $369
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pMiR-122a-5p-Luc LR-0018 $369
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pMiR-125a-5p-Luc LR-0019 $369
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pMiR-125b-5p-Luc LR-0020 $369
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pMiR-127-5p-Luc LR-0021 $369
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pMiR-132-3p-Luc LR-0022 $369
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pMiR-144-3p-Luc LR-0023 $369
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pMiR-146a-5p-Luc LR-0024 $369
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pMiR-155-5p-Luc LR-0025 $369
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pMiR-181a-5p-Luc LR-0026 $369
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pMiR-194-5p-Luc LR-0027 $369
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pMiR-196a-5p-Luc LR-0028 $369
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pMiR-199a-5p-Luc LR-0029 $369
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pMiR-206-Luc LR-0030 $369
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pMiR-214-3p-Luc LR-0031 $369
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pMiR-221-3p-Luc LR-0032 $369
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pMiR-222-3p-Luc LR-0033 $369
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pMiR-372-3p-Luc LR-0035 $369
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pMiR-373-5p-Luc LR-0036 $369
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pLet7a-Luc LR-0037 $369
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pLet7c-Luc LR-0038 $369
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pLet7d-Luc LR-0039 $369
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pLet7e-Luc LR-0040 $369
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pLet7f-Luc LR-0041 $369
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pLet7g-Luc LR-0042 $369
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pLet7i-Luc LR-0043 $369
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pMiR-1-Luc LR-0044 $369
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pMiR-10a-5p-Luc LR-0045 $369
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pMiR-10b-5p-Luc LR-0046 $369
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pMiR-143-3p-Luc LR-0047 $369
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pMiR-145-5p-Luc LR-0048 $369
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pMiR-15b-5p-Luc LR-0049 $369
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pMiR-181b-5p-Luc LR-0050 $369
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pMiR-18a-5p-Luc LR-0051 $369
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pMiR-19b-3p-Luc LR-0053 $369
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pMiR-9-5p-Luc LR-0054 $369
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pMiR-205-5p-Luc LR-0055 $369
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pMiR-208a-3p-Luc LR-0056 $369
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pMiR-34b-5p-Luc LR-0057 $369
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pMiR-34c-5p-Luc LR-0058 $369
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pMiR-210-3p-Luc LR-0059 $369
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pMiR-451a-Luc LR-0060 $369
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pMiR-106b-3p-Luc LR-0061 $369
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pMiR-18b-5p-Luc LR-0062 $369
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pMiR-29b-3p-Luc LR-0063 $369
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pMiR-153-3p LR-0064 $369
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pMiR-195-5p LR-0065 $369
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pMiR-223-3p LR-0066 $369
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(mum)pMiR-380-5p-Luc LR-0067 $369
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pMiR-496a-3p LR-0068 $369
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pMiR-448 LR-0069 $369
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pMiR-885-5p LR-0070 $369
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pMiR-455-5p LR-0071 $369
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pMiR-208b-3p LR-0072 $369
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pMiR-200c-3p LR-0073 $369
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pMiR-29c-3p LR-0074 $369
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pMiR-28-Luc LR-0075 $369
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pMiR-122-Luc LR-0076 $369
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pMiR-141-Luc LR-0077 $369
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pMiR-198-Luc LR-0078 $369
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pMiR-200-Luc LR-0079 $369
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pMiR-320-Luc LR-0080 $369
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pMiR-349-Luc LR-0081 $369
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pMiR-451-Luc LR-0082 $369
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pMiR-655-Luc LR-0083 $369
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pMiR-181-Luc LR-0084 $369
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pMiR-101a-Luc LR-0085 $369
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pMiR-200a-Luc LR-0086 $369
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pMiR-19-5p-Luc LR-0087 $369
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pMiR-27a-Luc LR-0088 $369
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pMiR-30b-Luc LR-0089 $369
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pMiR-380-Luc LR-0090 $369
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pMiR-885-Luc LR-0091 $369
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pMiR-99a-Luc LR-0092 $369
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pMiR-Luc-Control LR-1000 $369
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miRNA Luciferase Target Reporter Vectors

 

miRNAs have been shown to regulate many developmental and physiological processes, and the deregulation of miRNAs has been linked to a number of disease processes, including cancer. miRNAs play important roles in fine-tuning gene expression regulators referred to as “dimmer switches” because of their ability to repress gene expression without completely silencing it.  The precise monitoring of miRNA expression particularly in cell-based assay, can help to reveal the potential miRNA impacts on cancer etiology, differential stages and therapy development.  Signosis developed the miRNA target reporter vectors.  Each target reporter vector was constructed by inserting miRNA 3’UTR (~1kb) as corresponding miRNA binding sites on the downstream of luciferase reporter gene.  When miRNA in cells binds to the target site at 3'UTR downstream of reporter luciferase gene, it leads to repression of the expression of luciferase and results in reduction of luciferase enzyme activity. The miRNA expression level quantitatively corresponds with the luciferase activities.

 

 

Benefits:
  • Functional assay Monitor the  regulation of target genes by miRNA.
  • Quantitative analysis - Expression and activation of miRNAs are measured through suppression of firefly luciferase gene expression.
Product Name Catalog # Price   Qty
pLuc3U-BCL2 LR-1001 $459
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pLuc3U-Cox2 LR-1002 $459
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pLuc3U-DNMT3B LR-1003 $459
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pLuc3U-E2F1 LR-1004 $459
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pLuc3U-E2F3 LR-1005 $459
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pLuc3U-EGFR LR-1006 $459
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pLuc3U-ERa LR-1007 $459
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pLuc3U-Fos LR-1008 $459
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pLuc3U-HNF1A LR-1009 $459
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pLuc3U-HOXD10 LR-1010 $459
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pLuc3U-KRAS LR-1011 $459
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pLuc3U-PTEN LR-1012 $459
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pLuc3U-RB LR-1013 $459
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pLuc3U-TCL1 LR-1014 $459
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pLuc3U-TPM1 LR-1015 $459
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pLuc3U-TSP1 LR-1016 $459
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pLuc3U-Twist LR-1017 $459
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miRNA Luciferase Vector Sets

Benefits:
  • Functional assay It provides functional assays to monitor miRNA expression in cells.
  • Quantitative analysis - Expression and activity of miRNAs are measured through repression of firefly luciferase gene expression.
  • Comprehensive Moniter 7 miRNAs in one assay.
Product Name Catalog # Price   Qty
Oxidative Stress miRNA Reporter Vector Set LR-4001-50 $829
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Oxidative Stress miRNA Reporter Vector Set LR-4001-2000 $1,649
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Genotoxic Stress Luciferase Reporter Vector Set LR-4002-50 $829
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Genotoxic Stress Luciferase Reporter Vector Set LR-4002-2000 $1,649
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miRNA Plate Assays

With the Plate Assay kits, the conversion of miRNA into cDNA is not needed. It is 100 times more sensitive than Northern Blot Assay, and the discrimination power of distinguishing the isomers of miRNAs is also higher.

Benefits: 
  • Simple - Do miRNA detection as doing ELISA. It does not need enzymatic conversion from miRNA into cDNA.  The entire assay procedure is “incubation and wash”.
  • Higher sensitivity and better discrimination power - The detection is approximately 100 times more sensitive than miRNA Northern blot.  In addition, it has higher discrimination power than miRNA Northern blot in differentiating miRNA isoforms.
  • Quantitative comparison - The difference of two or more samples can be quantitatively analyzed.
Product Name Catalog # Price   Qty
miRNA Plate Assay Kit (Use with MO-XXXX) MA-0101 $419
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miR-17-92 Cluster Plate Assay MA-0102 $479
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Cancer miRNA Profiling Plate Assay I MA-0103 $479
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miR-21 Plate Assay MA-0104 $429
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Breast Cancer miRNA Plate Assay Kit I MA-0105 $479
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Breast Cancer miRNA Plate Assay Kit II MA-0106 $479
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Breast Cancer miRNA Plate Assay Kit III MA-0107 $479
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Lung Cancer miRNA Plate Assay I MA-0108 $479
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Colorectal Cancer miRNA Plate Assay I MA-0109 $479
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Colorectal Cancer miRNA Plate Assay II MA-0110 $479
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Colorectal Cancer miRNA Plate Assay III MA-0111 $479
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Colorectal Cancer miRNA Plate Assay IV MA-0112 $479
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Liver Cancer miRNA Plate Assay I MA-0113 $479
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Liver Cancer miRNA Plate Assay II MA-0114 $479
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Pancreatic Cancer miRNA Plate Assay I MA-0115 $479
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Pancreatic Cancer miRNA Plate Assay II MA-0116 $479
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Bladder Cancer miRNA Plate Assay I MA-0117 $479
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Ovarian Cancer miRNA Plate Assay I MA-0118 $479
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Ovarian Cancer miRNA Plate Assay II MA-0119 $479
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Ovarian Cancer miRNA Plate Assay III MA-0120 $479
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Prostate Cancer miRNA Plate Assay I MA-0121 $479
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Thyroid Cancer miRNA Plate Assay I MA-0122 $479
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Thyroid Cancer miRNA Plate Assay II MA-0123 $479
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Lymphoma miRNA Plate Assay I MA-0124 $479
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Leukemia miRNA Plate Assay I MA-0125 $479
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Leukemia miRNA Plate Assay III MA-0126 $479
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Leukemia miRNA Plate Assay IV MA-0127 $479
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Leukemia miRNA Plate Assay V MA-0128 $479
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Stem Cell-Specific miRNA Plate Assay Kit I MA-0129 $479
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Stem Cell-Specific miRNA Plate Assay Kit II MA-0130 $479
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Stem Cell-Specific miRNA Plate Assay Kit IV MA-0131 $479
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Lung Cancer miRNA Plate Assay II MA-0132 $479
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Lymphoma miRNA Plate Assay II MA-0133 $479
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Leukemia miRNA Plate Assay II MA-0134 $479
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Stem Cell-Specific miRNA Plate Assay Kit III MA-0135 $479
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Direct Hybridization Plate Assays

48 miRNAs can be quantitatively analyzed simultaneously via direct hybridization of RNA sample without any addition steps. The procedure is easy, and the conversion of the miRNA into a cDNA is not needed.

Benefits:
  • Simple Do miRNA detection as doing ELISA. It does not need enzymatic conversion from miRNA into cDNA. The entire assay procedure is “incubation and wash”.
  • Higher sensitivity and better discrimination power - The detection is approximately 100 times more sensitive than miRNA Northern blot. In addition, it has higher discrimination power than miRNA Northern blot in differetiating miRNA isoforms.
  • Quantitative comparison The difference of two or more samples in the expression of a specific miRNA can be quantitatively analyzed.
Product Name Catalog # Price   Qty
miRNA Direct Hybridization Plate Array I MA-1001 $439
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Apoptosis-Associated miRNA Plate Array MA-1002 $439
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Stem Cell-Associated miRNA Plate Array MA-1003 $439
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