Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based assays. Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis. However, because establishment of such cell lines are tedious in procedures, time-consuming in development process, and expensive in terms of labor, reagents, and materials, as well as post-transfection validation of individual clones, it is always debatable whether is worthy of developing such stable cell lines in individual laboratories.  

Signosis now provides a series of gene expression- or luciferase reporter-ready stable cell lines in order to facilitate the studies.

 

Luciferase Reporter Stable Cell Lines

Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based assays.  Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis.  At Signosis, we help researchers save time and labor in generating and validating stable cell lines so scientists can spend more effort on solving the big questions.  We offer a wide-selection of validated luciferase reporter cell lines that measure transcription factor activity as a read-out for various signaling pathways.

Benefits:
  • Routine mycoplasma testing - All cell lines tested negative for mycoplasma.
  • High sensitive and responsive - Each cell line is validated to induce strong reporter signal in response to stimuli.
  • Consistent - TF reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
  • Time saving - Cell line can be used for experiments right away to study different signaling pathways.
  • Firefly Luciferase SubstrateClick here to learn more about Signosis luciferase substrate.

Product Name Catalog # Price (FP)* Catalog # Price (NP)**   Qty
NFkB Luciferase Reporter HeLa Stable Cell Line SL-0001-FP $2,000 SL-0001-NP $950
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NFkB Luciferase Reporter NIH 3T3 Stable Cell Line SL-0006-FP $2,000 SL-0006-NP $950
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NFkB Luciferase Reporter HEK293 Stable Cell Line SL-0012-FP $2,000 SL-0012-NP $950
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NFkB Luciferase Reporter MCF-7 Stable Cell Line SL-0013-FP $3,000 SL-0013-NP $1,000
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NFkB Luciferase Reporter A549 Stable Cell Line SL-0014-FP $2,000 SL-0014-NP $950
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NFkB Luciferase Reporter HepG2 Stable Cell Line SL-0017-FP $2,000 SL-0017-NP $950
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NFkB Luciferase Reporter MEF Stable Cell Line SL-0033-FP $2,000 SL-0033-NP $950
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NFkB Luciferase Reporter Neuro2a Stable Cell Line SL-0026-FP $2,000 SL-0026-NP $950
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NFkB Luciferase Reporter Jurkat Stable Cell Line SL-0050-FP $3,000 SL-0050-NP $1,200
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NFkB Luciferase Reporter MDA-MB-231 Stable Cell Line SL-0043-FP $2,000 SL-0043-NP $950
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NFAT Luciferase Reporter Hela Stable Cell Line SL-0018-FP $2,000 SL-0018-NP $950
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NFAT Luciferase Reporter NIH 3T3 Stable Cell Line SL-0029-FP $2,000 SL-0029-NP $950
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NFAT Luciferase Reporter Jurkat T Stable Cell Line SL-0032-FP $2,000 SL-0032-NP $950
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p53 Luciferase Reporter RKO Stable Cell Line SL-0007-FP $3,000 SL-0007-NP $1,200
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p53 Luciferase Reporter Hela Stable Cell Line SL-0011-FP $3,000 SL-0011-NP $1,000
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SMAD/TGFbeta Luciferase Reporter HepG2 Stable Cell Line SL-0016-FP $3,000 SL-0016-NP $1,000
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SMAD/TGFbeta Luciferase Reporter NIH/3T3 Stable Cell Line SL-0030-FP $2,000 SL-0030-NP $950
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BMP Responsive Luciferase Reporter HEK293 Cell Line SL-0051-FP $3,000 SL-0051-NP $1,200
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NRF2/ARE Luciferase Reporter HEK293 Stable Cell Line SL-0042-FP $2,000 SL-0042-NP $950
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NRF2/ARE Luciferase Reporter HepG2 Stable Cell Line SL-0046-FP $2,000 SL-0046-NP $950
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NRF2/ARE Luciferase Reporter NIH3T3 Stable Cell Line SL-0047-FP $2,000 SL-0047-NP $950
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NRF2/ARE Luciferase Reporter MCF7 Stable Cell Line SL-0010-NP $1,200
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Stat3 Luciferase Reporter Hela Stable Cell Line SL-0003-FP $2,000 Sl-0003-NP $950
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Stat1 Luciferase Reporter Hela Stable Cell Line SL-0004-FP $2,000 SL-0004-NP $950
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HIF Luciferase Reporter NIH3T3 Stable Cell Line SL-0005-FP $2,000 SL-0005-NP $950
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HIF Luciferase Reporter Hela Stable Cell Line SL-0023-FP $2,000 SL-0023-NP $950
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HIF Luciferase Reporter Neuro2a Stable Cell Line SL-0027-FP $2,000 SL-0027-NP $950
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HIF Luciferase Reporter Cos-7 Stable Cell Line SL-0034-FP $2,000 SL-0034-NP $950
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Estrogen Receptor Luciferase Reporter T47D Stable Cell Line SL-0002-FP $2,000 SL-0002-NP $950
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AR Luciferase Reporter MDA-MB-453 Stable Cell Line SL-0008-FP $3,000 SL-0008-NP $1,000
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GR Luciferase Reporter MDA-MB-453 Stable Cell Line SL-0009-FP $3,000 SL-0009-NP $1,000
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GR Luciferase Reporter Hela Stable Cell Line SL-0021-FP $3,000 SL-0021-NP $1,000
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AP-1 Luciferase Reporter Hela Stable Cell Line SL-0019-FP $2,000 SL-0019-NP $950
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CREB Luciferase Reporter HEK293 Stable Cell Line SL-0020-FP $2,000 SL-0020-NP $950
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CREB Luciferase Reporter NIH 3T3 Stable Cell Line SL-0031-FP $2,000 SL-0031-NP $950
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CREB Luciferase Reporter HCC827 Stable Cell Line-np SL-0057-FP $1,500 SL-0057-np $750
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TCF/LEF Luciferase Reporter HEK293 Stable Cell Line SL-0015 FP $3,000 SL-0015 NP $1,200
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TCF/LEF Luciferase Reporter Hela Stable Cell Line SL-0022-FP $2,000 SL-0022-NP $950
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TCF/LEF Luciferase Reporter CHO-K1 Stable Cell Line SL-0028-FP $2,000 SL-0028-NP $950
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IRF Luciferase Reporter HEK293 Stable Cell Line SL-0035-FP $2,000 SL-0035-NP $950
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IRF Luciferase Reporter HepG2 Stable Cell Line SL-0049-FP $2,000 SL-0049-NP $950
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ATF6 Luciferase Reporter CHO-K1 Stable Cell Line SL-0024-FP $2,000 SL-0024-NP $950
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CHOP Luciferase Reporter Stable Cell Line SL-0025-FP $2,000 SL-0025-NP $950
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Control Hela Luciferase Reporter Stable Cell Line SL-0038-FP $800 SL-0038-NP $800
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Control HepG2 Luciferase Reporter Stable Cell Line SL-0039 $800
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ELK-TAD Luciferase Reporter HEK293 Stable Cell Line SL-0040-FP $2,000 SL-0040-NP $950
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ELK-TAD Luciferase Reporter Hela Stable Cell Line SL-0041-FP $2,000 SL-0041-NP $950
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IL-6 Responsive Luciferas Reporter HEK293 Cell Line SL-0048-FP $3,000 SL-0048-NP $1,200
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IFN-a/ISRE Luciferase Reporter HeLa Stable Cell Line SL-0052-FP $2,000 SL-0052-NP $950
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MRF Luciferase Reporter HEK293 Stable Cell Line SL-0053-FP $2,000 SL-0053-NP $950
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FXR Luciferase Reporter HepG2 Stable Cell Line SL-0055-FP $3,000 SL-0055-NP $1,200
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SRF Luciferase Reporter HEK293 Stable Cell Line SL-0056-FP $2,000 SL-0056-NP $950
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  • * For Profit (FP) price is for non-academic, private companies and institutes
  • ** Non Profit (NP) price is for academic, non profit organizations and institutes

NR LBD-Driven GAL4 Reporter Stable Cell Lines

Nuclear receptors (NRs) are a superfamily of transcription factors that regulate key aspects of development, metabolism and disease. In  general,  they  activate  the transcription  of  sets  of  genes  in  response  to  the binding  of  cognate  ligands. The classic modular domain organization of NRs includes a variable N-terminal domain (NTD), a centrally located zinc-finger DNA-binding domain (DBD) and a C-terminal  ligand-binding  domain  (LBD).

The GAL4-UAS system is a powerful technique for studying gene expression and function. It has also been adapted to study nuclear receptor chemical-binding functions. Signosis NR LBD-driven GAL4 reporter stable cell lines is a reliable, rapid, and sensitive method for analyzing the intracellular status of NRs upon exposure to drug candidates or other stimuli. These functional assays allow for measurement of receptor activation or inhibition by compounds and can be used to determine compound potency and selectivity.

 

Benefits:

  • Highly specific–the responsiveness of this stable cell line is driven by the specific ligand binding domain with low/no cross reactivity with other members of nuclear receptor.
  • Less toxic – The chimeric receptors display less toxic effects on the cells.
  • Highly sensitive – More than 50 fold induction in response to the corresponding stimuli.
  • Routine mycoplasma testing - All cell lines tested negative for mycoplasma.

 

Product Name Catalog # Price (FP)* Catalog # Price (NP)**   Qty
GR LBD-driven GAL4 reporter HEK 293 stable cell line SL-3001-FP $6,000 SL-3001-NP $3,000
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  • * For Profit (FP) price is for non-academic, private companies and institutes
  • ** Non Profit (NP) price is for academic, non profit organizations and institutes

EGFR Stably Expressing Cell Lines

EGFR mutations occur in many type of cancers including non-small cell lung cancer. Common mutations found in these patients are deletions in exon 19 (Del19) and L858R substitution in exon 21.  Patients with these mutations are sensitive to first (gefitinib or erlotinib) and second (afatinib) generation EGFR tyrosine kinase inhibitors (TKIs) , whereas patients with wild type EGFR are not.  However, eventually all patients become resistant to first and/or second generation EGFR TKIs and acquired T790M secondary mutation in exon 20 accounts for more than 50% of the acquired resistance.  Cells expressing EGFR with either L858R+ T790M or Del19+T790M double mutations are resistant to induced apoptosis in the presence of first or second generation EGFR TKIs. 

Eight cell lines stably expressing HA-tagged EGFR have been established in the IL3-dependent Ba/F3 cell lines —WT EGFR, L858R EGFR mutant, L858R/T790M EGFR double mutant, Del19 (del747-752) EGFR mutant, Del19/T790M EGFR mutant, three ins20 (D770_N771insSVD,A763_Y764insFQEA, A767_dupASV) EGFR mutants —and Ba/F3 cell line expressing empty vector.  Also, two cell lines stably expressing HA-tagged EGFR have been established in HCC827 cell lines —Exon19del EGFR mutant, Exon19del/T790M EGFR double mutant—and HCC827 cell line expressing empty vector.

Due to their proliferation upon EGFR activation, sensitivity to TKIs, or resistance to induced apoptosis, these cell lines can be used to study the molecular mechanism underlying susceptibility of tumors to the drugs as well as screening and validating new TKIs.

Product Name Catalog # Price (FP)* Catalog # Price (NP)**   Qty
Control (Empty Vector) Ba/F3 Stable Cell Line EL-001-FP $3,000 EL-001-NP $1,200
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EGFR (Wild-type) Stably Expressing Ba/F3 Cell Line EL-002-FP $3,000 EL-002-NP $1,200
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EGFR (L858R) Stably Expressing Ba/F3 Cell Line EL-003-FP $3,000 EL-003-NP $1,200
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EGFR (L858R+T790M) Stably Expressing Ba/F3 Cell Line EL-004-FP $3,000 EL-004-NP $1,200
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EGFR( D770_N771insSVD )stably Expressing BaF3 stable cell line EL-008-FP $3,000 EL-008-NP $1,200
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EGFR (A763_Y764insFQEA) stably Expressing BaF3 cells EL-009-FP $3,000 EL-009-NP $1,200
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EGFR (A767_dupASV) stably expressing BaF3 cells EL-010-FP $3,000 EL-010-NP $1,200
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EGFR (DEL19) stably expressing BaF3 cells EL-011-FP $3,000 EL-011-NP $1,200
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EGFR (Del19-T790M) stably expressing BaF3 stable cell line EL-014-FP $3,000 EL-014-NP $1,200
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EGFR (E19del) Stably Expressing HCC827 Cell Line EL-005-FP $3,000 EL-005-NP $1,200
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EGFR (E19del+T790M) Stably Expressing HCC827 Cell Line EL-006-FP $3,000 EL-006-NP $1,200
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Control (Empty Vector) HCC827 Stable Cell Line EL-007-FP $3,000 EL-007-NP $1,200
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EGFR L858R Stably Expressing CHO Cell Line EL-016-FP $3,000 EL-016-NP $1,200
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EGFR T790M Stably Expressing CHO Cell Line EL-017-FP $3,000 EL-017-NP $1,200
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EGFR T790M Stably Expressing 293 Cell Line EL-018-FP $3,000 EL-018-NP $1,200
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  • * For Profit (FP) price is for non-academic, private companies and institutes
  • ** Non Profit (NP) price is for academic, non profit organizations and institutes

EGFR Mutant and WT Expression Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death.  Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand binding domain and exons 18–24 code for the TK domain.  Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT and JAK/STAT pathways which can lead to cell survival, proliferation and migration of cancer cells.

 

 

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.

 

Highlight

  • Panel of EGFR Mutant Vectors - The panel includes more than 12 ready to go EGFR mutants.
  • DYKDDDDK-tag for easy detection - This tag is engineered to downstream of EGFR gene to facilitate Western blot
  • perfect for stable cell line establishment - The hygromycin resistant gene is embedded in the vector for stable cell line establishment.
  • Custom service available - We also offer the custom service with the same list price. If you have any mutants in your mind, please let us know.

 

 

Product Name Catalog # Price   Qty
EGFR (WT) EV-0001 $1,200
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EGFR (G719S) EV-0002 $1,200
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EGFR (G719A) EV-0003 $1,200
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EGFR (G719C) EV-0004 $1,200
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EGFR (T790M) EV-0005 $1,200
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EGFR (L858Q) EV-0006 $1,200
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EGFR (L858R) EV-0007 $1,200
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EGFR Exon 19 Deletion EV-0008 $1,200
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EGFR (L861Q) EV-0009 $1,200
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EGFR Exon 19 Insertion EV-0010 $1,200
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EGFR Exon 20 Insertion EV-0011 $1,200
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EGFR (C797S) EV-0012 $1,200
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EGFR (T790M C797S) EV-0013 $1,500
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EGFR (T790M C797S Exon 19 Deletion) EV-0014 $1,500
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Parental Cell Lines

Signosis offers some of the parental cells used for generating its stable cell lines. These cell lines can be used as a match control for their related stable cell lines. All cell lines tested negative for mycoplasma.

Product Name Catalog # Price   Qty
Hela Cells PC-001 $300
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HEK293 PC-002 $300
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A549 Cells PC-003 $300
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NIH/3T3 PC-004 $300
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HepG2 PC-005 $300
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Jurkat Cells PC-006 $300
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K562 Cells PC-007 $300
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MCF7 PC-008 $300
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T47D PC-009 $300
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COS-7 PC-010 $300
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SF9 PC-011 $300
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Bioluminescent Tumor Cell Lines

Bioluminescent tumor cell lines are critical to cancer investigation, especially imaging of tumors in xenografted animals.  They are used for assessing dissemination of tumor cells in the body of xenografted mice as well as tumor growth or regression under treatment.  Although various types of reporter genes encoding fluorescent proteins have been reported, stable expression of exogenous luciferase in tumor cells combined to systemic injection of luciferin provides an excellent signal/background ratio for external optical imaging.  Signosis has generated a number of luciferase-positive tumor cell lines representative of multiple tumor phenotypes, facilitating evaluation of novel therapeutic modalities.

In addition, bioluminescent tumor cell lines can be used to develop a luciferase activity-based assay, as a positive control for luciferase activity, or to test if experimental conditions affect luciferase expression

Benefits:
  • Consistent - Exogenous gene is stably integrated into the genome to avoid experimental/cell-to-cell variations.
  • Time saving - Cell line can be used for experiments right away to study different signaling pathways.
Product Name Catalog # Price (FP)* Catalog # Price (NP)**   Qty
Luciferase Stably Expressing K562 Cell Line ( ) SL-0106-FP $1,200 SL-0106-NP $600
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Luciferase Stably Expressing HEK293 Cell Line SL-0103-FP $1,200 SL-0103-NP $600
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Luciferase Stably Expressing Hela Cell Line SL-0102-FP $1,200 SL-0102-NP $600
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Luciferase Stably Expressing Jurkat Cell Line SL-0036-FP $1,200 SL-0036-NP $600
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Luciferase Stably Expressing NIH/3T3 Cell Line SL-0045-FP $1,200 SL-0045-NP $600
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Luciferase Stably Expressing A549 Cell Line SL-0104-FP $1,200 SL-0104-NP $600
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Luciferase Stably Expressing HepG2 Cell Line ( ) SL-0105-FP $1,200 SL-0105-NP $600
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  • * For Profit (FP) price is for non-academic, private companies and institutes
  • ** Non Profit (NP) price is for academic, non profit organizations and institutes

Stably Expressing Cell Lines

Product Name Catalog # Price (FP)* Catalog # Price (NP)**   Qty
Control (Empty Vector) Ba/F3 Stable Cell Line EL-001-FP $3,000 EL-001-NP $1,200
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EGFR (Wild-type) Stably Expressing Ba/F3 Cell Line EL-002-FP $3,000 EL-002-NP $1,200
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EGFR (L858R) Stably Expressing Ba/F3 Cell Line EL-003-FP $3,000 EL-003-NP $1,200
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EGFR (L858R+T790M) Stably Expressing Ba/F3 Cell Line EL-004-FP $3,000 EL-004-NP $1,200
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EGFR (E19del) Stably Expressing HCC827 Cell Line EL-005-FP $3,000 EL-005-NP $1,200
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EGFR (E19del+T790M) Stably Expressing HCC827 Cell Line EL-006-FP $3,000 EL-006-NP $1,200
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Control (Empty Vector) HCC827 Stable Cell Line EL-007-FP $3,000 EL-007-NP $1,200
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Tet-OFF Control Overexpression MCF7 Stable Cell Line EL-012-FP $3,000 EL-012-NP $1,200
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Notch ICD Tet-OFF Overexpression MCF7 Stable Cell Line EL-013-FP $3,000 EL-013-NP $1,200
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GSK3B Stably Expressing Hela Cell Line EL-1001-FP $2,000 EL-1001-NP $950
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GSK3B Stably Expressing HEK293 Cell Line EL-1002-FP $2,000 EL-1002-NP $950
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MMP9 Stably Expressing HEK293 Cell Line EL-1003-FP $2,000 EL-1003-NP $950
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P53 Stably Expressing Cell Line SL-0100-FP $1,200 SL-0100-NP $950
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NFκB (p65) Stably Expressing Cell Line SL-0101-FP $1,200 SL-0101-NP $950
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  • * For Profit (FP) price is for non-academic, private companies and institutes
  • ** Non Profit (NP) price is for academic, non profit organizations and institutes